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Recherche de bacteries thermophiles resistantes aux antibiotiques dans les bains traditionnels |
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| Authors: | Fouzia Rhazi Filali Abdelhamid Zaid Zoubir Zekhnini Jean-Marie Frere |
| Institution: | 1 Département de Biologie, Faculté des Sciences, Université My Ismaïl B.P. 4010, Zitoune, Meknès, Maroc 2 Centre d'Ingégnierie des Protéines, Université de Liège, Institut de chimie, B6-4000, Sart Tilman (Liège 1), Belgique |
| Abstract: | Three thermophilic bacteria strains, designated strain BS1, BS2 and BS3, resistant to β-lactam antibiotics, and leaving at an optimal temperature for growth of about 50°C, were isolated from traditional baths in Meknes-city in Morocco. Physiological and biochemical studies showed that these organisms belong to Gram positive Bacilli. They could not be identified with the Bergey's Manuel of Systematic Bacteriology (1986). The dosage of β-lactamase during the exponential growth phase has revealed that the strain BS3 produces a maximal amount of this enzyme. Studies aimed at determining the optimal conditions for incubation and growth have been performed in order to optimize the excretion of β-lactamase by BS3 cells and thus facilitate the purification and and characterization of this enzyme. |
| Keywords: | Bacillus thermophiles antibiotics β-lactamase |
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