| 一株桑树内生拮抗细菌的分离鉴定与抑菌活性 |
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| 引用本文： | 谭广秀,吴琼英,贾俊强,桂仲争,杜金娟,陈炼.一株桑树内生拮抗细菌的分离鉴定与抑菌活性[J].蚕业科学,2012(3):412-417. |
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| 作者姓名： | 谭广秀 吴琼英 贾俊强 桂仲争 杜金娟 陈炼 |
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| 作者单位： | 江苏科技大学生物与化学工程学院;江苏科技大学蚕业研究所 |
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| 基金项目： | 江苏科技大学青年骨干教师支持计划项目(No.37210901) |
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| 摘 要： | 以大肠杆菌为指示菌,从野生型鲁桑(Morus multicaulis Perr.)桑树枝条韧皮部组织中分离得到具有抑菌活性的内生细菌ME1。形态与生理生化特性鉴定ME1菌株为有芽孢和鞭毛的革兰阳性菌,细胞呈杆状,大小为0.6～0.7μm×1.8～1.9μm;能够利用葡萄糖、蔗糖和乳糖等碳水化合物,所产淀粉酶活力可达4 182.60 U/g;耐盐性较高,可耐受100 g/L NaCl。进一步通过16S rDNA序列及其系统进化分析,初步鉴定ME1菌株为枯草芽孢杆菌(Bacillus subtilis)。抑菌试验表明ME1菌株的代谢产物具有广谱抗菌活性,对大肠杆菌(Escherichia coli)、苏云金芽孢杆菌(Bacillus thuringiensis)、金黄色葡萄球菌(Staphylococcus aureus)、蕈状芽孢杆菌(Bacillus mycoides)和白僵菌(Beauveria bassiana)的体外抑制率分别为59.92%、75.70%、51.60%、87.25%和69.81%,该菌株产生的抗菌活性物质具有潜在的医用开发价值。
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| 关 键 词： | 桑树内生细菌 形态特征 生理生化特性 16SrDNA序列 抑菌活性 |
Isolation,Identification and Antibacterial Activity of an Endophytic Bacterial Strain in Mulberry |
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| TAN Guang-Xiu,WU Qiong-Ying,JIA Jun-Qiang,GUI Zhong-Zheng,DU Jin-Juan,CHEN Lian.Isolation,Identification and Antibacterial Activity of an Endophytic Bacterial Strain in Mulberry[J].Acta Sericologica Sinica,2012(3):412-417. |
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| Authors: | TAN Guang-Xiu WU Qiong-Ying JIA Jun-Qiang GUI Zhong-Zheng DU Jin-Juan CHEN Lian |
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| Institution: | 1(1School of Biology and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China;2The Sericultural Research Institute,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China) |
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| Abstract: | Using Escherichia coli as the indicator bacterium,an endophytic bacterial strain(designated as ME1) with antibacterial activity was isolated from the phloem tissue of Morus multicaulis Perr.twig.Morphology,physiological and biomedical characteristics identification tests revealed that ME1 was a Gram positive bacterium with flagellum and spore,was rod-shaped and 0.6~0.7 μm×1.8~1.9 μm in size.ME1 could produce amylase with activity as high as 4 182.60 U/g,being able to use carbohydrates such as glucose,sucrose,and lactose.Moreover,ME1 was able to withstand high salt stress,being endurable to 100 g/L NaCl.ME1 was preliminarily identified as Bacillus subtilis through further analyses to its 16S rDNA sequence and phylogenesis.Antagonism test showed that metabolites of ME1 had wide antibacterial spectrum.The inhibition rate to Escherichia coli,Bacillus thuringiensis,Staphylococcus aureus,Bacillus mycoides and Beauveria bassiana reached 59.92%,75.70%,51.60%,87.25% and 69.81%,respectively.This high antibacterial substance produced by ME1 has potential value for development of medical agents. |
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| Keywords: | Mulberry endophytic bacterium Morphological characteristic Physiological and biochemical properties 16S rDNA sequence Antibacterial activity |
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