| 昆明鼠超数排卵影响因素研究 |
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| 引用本文： | 崔元戎,金辉,杜春艳,王纯耀,陈辉,张钦宪.昆明鼠超数排卵影响因素研究[J].动物医学进展,2012(2):55-57. |
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| 作者姓名： | 崔元戎 金辉 杜春艳 王纯耀 陈辉 张钦宪 |
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| 作者单位： | 郑州大学医学院细胞生物学与医学遗传学教研室;郑州大学医学院组织胚胎学教研室;郑州大学实验动物中心 |
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| 基金项目： | 河南省重大科技攻关计划资助项目(0623031400) |
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| 摘 要： | 为探讨昆明鼠超数排卵的最佳条件,提高超排效果,比较促性腺激素使用剂量、孕马血清促性腺激素(PMSG)与人绒毛膜促性腺激素(hCG)注射间隔时间、超排小鼠所处发情周期阶段及小鼠周龄等因素对昆明鼠超数排卵结果的影响。PMSG 8IU+hCG 8IU、间隔46h～48h注射组超排效果最好;3周龄、8周龄～10周龄雌鼠较4周龄～7周龄雌鼠超排效果好,小鼠发情间期或发情前期开始超排的效果显著高于发情周期的其他时期。
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| 关 键 词： | 昆明鼠 超数排卵 激素剂量 发情周期阶段 |
Effecting Factors’ Analysis of Superovulation in Kunming Mice |
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| Institution: | CUI Yuan-rong1,JIN Hui2,DU Chun-yan3,WANG Chun-yao3,CHEN Hui1,ZHANG Qing-xian2(1.Department of Cell Biology and Medical Genetics,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou,Henan, 450052,China;2.Department of Histology and Embryology,College of Basic Medical Sciences,Zhengzhou University, Zhengzhou,Henan,450052,China;3.Laboratory Animal Center of Zhengzhou University,Zhengzhou,Henan,450052,China) |
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| Abstract: | In order to study the best conditions of superovulation in Kunming mice,the results of superovulation in different conditions such as gonadotrophins dosage uesed,interval time of pregnant mare serum gonadotropin(PMSG) and human chorionic gonadotropin(hCG) injection,the stage of estrous cycle in the superovulated mice and mouse age etc were studied.The superovulation was best when PMSG 8 IU + hCG 8 IU were injected,and the interval was 46 h-48 h.The ova’s numbers of mice in 3 or 8-10 weeks by superovulation were more than that in 4-7 weeks,the superovulation results in estrus interphase of mice or prophase were better than that in other periods. |
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| Keywords: | Kunming mouse superovulation hormone dosage stage of estrous cycle |
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