| 低温预处理和取材部位对甘蓝花药愈伤组织诱导的影响 |
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| 引用本文： | 李建斌,王神云,季核亮,王红,万雁玲.低温预处理和取材部位对甘蓝花药愈伤组织诱导的影响[J].天津农学院学报,2010,17(3):14-16,20. |
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| 作者姓名： | 李建斌 王神云 季核亮 王红 万雁玲 |
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| 作者单位： | 1. 江苏省农业科学院,蔬菜研究所,南京210014
2. 天津市耕耘种业有限公司,天津,300400 |
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| 基金项目： | 江苏省科技支撑计划项目"优良甘蓝分子育种技术和新品种选育及其高效种植技术研究"，国家"863"计划项目"优质多抗蔬菜和果树分子育种技术与品种创制"，国家科技支撑计划项目"甘蓝雄性不育育种技术研究与新品种选育" |
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| 摘 要： | 以B475、B476、B323和B523 4个不同类型的甘蓝自交系为试材,研究了低温预处理、取材部位对甘蓝花药愈伤组织诱导培养的影响。结果表明,在花药培养前对花蕾进行4℃低温预处理0、1、2、3、4、5 d共6个处理中,4个甘蓝基因型均在低温预处理3 d时愈伤组织的诱导率较高,分别为42.77%、59.40%、71.80%和54.06%;对4个材料在同一生长时期不同部位的花蕾进行培养,花药愈伤组织诱导无显著差异。
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| 关 键 词： | 甘蓝 花药培养 低温预处理 取材部位 愈伤组织 |
Influences of Lower Temperature Pretreatment and Material Positions on Inducing Callus from Anther in Cabbage(Brassica oleracea var. capitata) |
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| LI Jian-bin,WANG Shen-yun,JI He-liang,WANG Hong,WAN Yan-ling.Influences of Lower Temperature Pretreatment and Material Positions on Inducing Callus from Anther in Cabbage(Brassica oleracea var. capitata)[J].Journal of Tianjin Agricultural College,2010,17(3):14-16,20. |
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| Authors: | LI Jian-bin WANG Shen-yun JI He-liang WANG Hong WAN Yan-ling |
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| Institution: | 1.Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.Tianjin Gengyun Seed.LTD,Tianjin 300400,China) |
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| Abstract: | Using genotypes B475,B476,B323 and B523 of cabbage（Brassica oleracea var.capitata）as the materials,the effects of lower temperature pretreatment and material positions on inducing callus of cabbage anther culture were studied.The results show that in the six treatments of fresh materials and with lower temperature pretreatment of 0,1,2,3,4,5 d,pretreating the four genotypes for 3 days had the highest induction frequency,and they are 42.77%,59.40%,71.80% and 54.06%,respectively.Materials gotten from different positions of the same plant at the same growth period had no significant influences on the induction culture. |
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| Keywords: | cabbage（Brassica oleracea var capitata） anther culture lower temperature pretreatment material position callus |
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