| 运用差异显示技术分离水花生在干旱胁迫下表达上调的基因 |
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| 引用本文： | 高建明,肖强,尹亮,何光源.运用差异显示技术分离水花生在干旱胁迫下表达上调的基因[J].农业科学与技术,2007,8(1):2-6. |
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| 作者姓名： | 高建明 肖强 尹亮 何光源 |
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| 作者单位： | 华中科技大学生命科学与技术学院中英联合实验室,湖北武汉430074 |
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| 摘 要： | 干旱发生时,水花生的须根将转变成又长又粗的肉质根,这有利于保水和从土壤深处汲取水分以减轻旱害.本研究中采用差异显示技术克隆干旱发生时与水花生根形态发生相关的基因,为进一步通过转基因提高农作物抗旱性奠定基础;共使用15对引物组合,回收到20个可能的在干旱诱导后表达上调的带,其中有一个能被反向northern所证实;而后对其进行了克隆和测序;同源性比对表明它可能是一个新基因的片段.半定量RT-PCR分析进一步证明该基因在干旱和盐胁迫下表达上调.
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| 关 键 词： | 干旱 差异显示 反向Northern印迹杂交 半定量RT-PCR drought differential display reverse northern blot semi-quantitative RT-PCR 运用 差异显示 技术分离 水花生 干旱胁迫 表达上调 基因 technique differential display genes drought salt stress analysis sequence reverse northern blot induced primer transgenic method |
Isolation of the drought up-regulated gene in Alternanthera philoxeroides by differential display technique |
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| Gao Jianming,Xiao Qiang,Yin Liang,He Guangyuan.Isolation of the drought up-regulated gene in Alternanthera philoxeroides by differential display technique[J].Agricultural Science ＆ Technology,2007,8(1):2-6. |
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| Authors: | Gao Jianming Xiao Qiang Yin Liang He Guangyuan |
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| Abstract: | Under drought stress, adventitious roots of Alternanthera philoxeroides seedlings will grow long thick fleshy roots, which are assumed to improve performance of the plant by more efficient reservation and absorption of water from deep soil layers. In this study, the differential display technique was used to clone morphogenesis-related genes from A. philoxeroides roots treated with drought, which would help to improve crop plants' drought-tolerance by transgenic method;by 15 pairs of primer combinations, twenty putative drought up-regulated gene segments induced by drought were obtained;and one of them was confirmed by reverse northern blot, and subsequently cloned and sequenced. A homologous analysis revealed that it might be a new sequence.Semi-quantitative RT-PCR analysis showed that the gene was up-regulated by drought and salt stress. |
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| Keywords: | drought differential display reverse northern blot semi-quantitative RT-PCR |
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