| 珠眉海棠(Malus zumi Mats)盐应答MzSTO基因的克隆与表达分析 |
| |
| 引用本文： | 蒋玉妆,李凌子,赵宇,李擎天,梁占锋,孔瑾.珠眉海棠(Malus zumi Mats)盐应答MzSTO基因的克隆与表达分析[J].中国农业大学学报,2013,18(1):88-93. |
| |
| 作者姓名： | 蒋玉妆 李凌子 赵宇 李擎天 梁占锋 孔瑾 |
| |
| 作者单位： | 中国农业大学 农学与生物技术学院,北京,100193 |
| |
| 基金项目： | 国家自然科学基金资助项目(31171940);北京市自然科学基金资助项目(6112012) |
| |
| 摘 要： | 通过微列阵芯片(Microarray)分析,从珠眉海棠盐胁迫cDNA文库中分离得到MzSTO (Salt tolerance protein)全长基因.结果表明:MzSTO基因cDNA全长1 066 bp,开放阅读框共729 bp,5'-UTR和3'-UTR的长度分别是49 bp和288 bp; MzSTO编码242个氨基酸,N端有2个保守的B-box锌指结构域(CX2CX8CX7CX2CX4HX8H)；半定量RT-PCR表明MzSTO基因受到盐和干旱胁迫抑制,受冷处理诱导,并响应ABA(abscisic acid).以上结果表明MzSTO基因可能通过依赖ABA的途径参与非生物逆境胁迫.
|
| 关 键 词： | 珠眉海棠 MzSTO 非生物胁迫 RT-PCR |
| 收稿时间： | 2012/4/17 0:00:00 |
Cloning and expression analysis of salt stress MzSTO gene from Malus zumi Mats |
| |
| JIANG Yu-zhuang,LI Ling-zi,ZHAO yu,LI Qing-tian,LIANG Zhan-feng and KONG Jin.Cloning and expression analysis of salt stress MzSTO gene from Malus zumi Mats[J].Journal of China Agricultural University,2013,18(1):88-93. |
| |
| Authors: | JIANG Yu-zhuang LI Ling-zi ZHAO yu LI Qing-tian LIANG Zhan-feng and KONG Jin |
| |
| Institution: | *(College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China) |
| |
| Abstract: | The full-length cDNA sequence of MzSTO gene was isolated from a cDNA library of Malus zumi.The full-length cDNA of MzSTO was 1 066 bp.The 5''-UTR and the 3''-UTR were 49 and 288 bp respectively.Its open reading frame of 729 bp encoded a protein of 242 amino acids.There were two B-box zinc-finger domains (CX2CX8CX7CX2CX4HX8H) near N-terminal.MzSTO was induced by low temperature stress and repressed by high-salinity and drought treatment,which was,also responsive to ABA (abscisic acid) treatment by semi-quantative RT-PCR.All the results showed that MzSTO was involved in response of abiotic stresses including salt, drought and cold stresses through a possible ABA-dependent pathway in Malus zumi. |
| |
| Keywords: | Malus zumi Mats MzSTO abiotic stresses RT-PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国农业大学学报》浏览原始摘要信息 |
| 点击此处可从《中国农业大学学报》下载免费的PDF全文 |
