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| 草地早熟禾种质资源遗传多样性的RAPD鉴定与分类研究 | |
| 引用本文： | 高丽娜,陈雅君,高丽娟,申凤娟.草地早熟禾种质资源遗传多样性的RAPD鉴定与分类研究[J].东北农业大学学报,2007,38(3):325-329. |
| 作者姓名： | 高丽娜 陈雅君 高丽娟 申凤娟 |
| 作者单位： | 1. 东北农业大学园艺学院,哈尔滨,150030;黑龙江东方学院艺术学部,哈尔滨,150086 2. 东北农业大学园艺学院,哈尔滨,150030 3. 大庆师范学院初等教育系,黑龙江,大庆,163712 |
| 摘 要： | 采用随机扩增多态性DNA(RAPD)标记,筛选出69个随机引物对32份草地早熟禾品种遗传谱带进行分析,46个引物共扩增出了197条扩增片断,其中多态性带数为195条,每个引物扩增出1～9条多态性带,平均每条引物扩增出4.3条。根据Jaccard相似系数分析RAPD结果,并利用NTSYS软件,按UPGMA平均法进行聚类。结果表明,第一大组群包括18个品种,第二大组群包括3个品种,第三大组群包括2个品种,第四大组群包括8个品种,第五大组群仅1个品种,为C_2。 |
| 关 键 词： | 草地早熟禾 遗传多样性 RAPD 聚类分析 |
| 文章编号： | 1005-9369（2007）03-0325-05 |
| 修稿时间： | 2006-03-24 |
Study on classification and genetic diversity of Kentucky bluegrasses by using RAPD markers |
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| GAO Lina,CHEN Yajun,GAO Lijuan,SHEN Fengjuan.Study on classification and genetic diversity of Kentucky bluegrasses by using RAPD markers[J].Journal of Northeast Agricultural University,2007,38(3):325-329. | |
| Authors: | GAO Lina CHEN Yajun GAO Lijuan SHEN Fengjuan |
| Institution: | 1. College of Horticulture, Northeast Agricultural University, Harbin 150030, China; 2. Art Department, Heilongjiang Oriental College, Harbin 150086, China; 3. Elementary Education Department, Daqing Normal University, Daqing Heilongjiang 163712, China |
| Abstract: | Genetic diversity of 32 Kentucky bluegrass cultivars was analyzed based on random amplified polymorphic DNA(RAPD) markers.A total of 197 bands were amplified,while 195 bands were polymorphic.One to nine polymorphic bands with an average of 4.3 could be amplified from each primer.The RAPD data of 32 cuhivars were analyzed and separated into different groups by Jaccard's coefficient and UPGMA clustering method with NTSYS software.Eighteen of the 32 were in group 1,8 of the 32 were in group 4.The remains were in other 3 groups. |
| Keywords: | RAPD |
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