| 硫化物胁迫对凡纳滨对虾血细胞的毒性影响 |
| |
| 引用本文： | 汪蕾,冼健安,张秀霞.硫化物胁迫对凡纳滨对虾血细胞的毒性影响[J].水生态学杂志,2017,38(3):97-102. |
| |
| 作者姓名： | 汪蕾 冼健安 张秀霞 |
| |
| 作者单位： | 华南师范大学药物研究院,中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所 |
| |
| 基金项目： | 国家自然科学基金(31500326), 广东省自然科学基金(2014A030310185), 广东省普通高校青年创新人才项目(2014KQNCX056),广东省普通高校特色创新项目(2015KTSCX019), 华南师范大学青年教师科研培育基金(13KJ16)。 |
| |
| 摘 要： | 硫化物是水产养殖过程中常见的水体污染物之一。为探讨水体硫化物胁迫对虾类血细胞的毒性影响,以不同浓度(0.5 mg/L,2.0 mg/L)的硫化物对凡纳滨对虾(Litopenaeus vannamei)进行胁迫,于胁迫后6、12、24和48 h取血淋巴,应用流式细胞术测定血细胞的总数(THC)、活性氧(ROS)含量、一氧化氮(NO)含量以及凋亡率。结果显示,经0.5 mg/L硫化物胁迫48 h后,对虾THC显著下降至11.78×10~6个/mL,为对照组的72.7%(P0.05),ROS含量为对照组的225.2%(P0.05),血细胞凋亡率显著上升至7.42%(P0.05);经2.0 mg/L硫化物胁迫6 h开始,对虾THC呈现显著的下降(P0.05),血细胞ROS含量和凋亡率显著提高(P0.05);表明硫化物胁迫刺激对虾血细胞产生大量ROS,诱导血细胞凋亡,从而导致THC下降,这一过程可能是硫化物导致虾类细胞免疫下降的重要机制;随着硫化物浓度的升高,其细胞毒性作用明显提高。血细胞NO含量在2.0 mg/L硫化物胁迫12和48 h时显著升高(P0.05),推测NO可能在硫化物胁迫防御调控中起着信号因子的作用。
|
| 关 键 词： | 硫化物 凡纳滨对虾 血细胞 凋亡 |
| 收稿时间： | 2016/4/7 0:00:00 |
| 修稿时间： | 2017/3/24 0:00:00 |
Toxic Effects of Sulfide on Haemocytes of Litopenaeus vannamei |
| |
| WANG Lei,XIAN Jian-an and ZHANG Xiu-xia.Toxic Effects of Sulfide on Haemocytes of Litopenaeus vannamei[J].Journal of Hydroecology,2017,38(3):97-102. |
| |
| Authors: | WANG Lei XIAN Jian-an and ZHANG Xiu-xia |
| |
| Institution: | Institute of Pharmaceutical Research, South China Normal University, Guangzhou 510631, P.R. China;,Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 57110, P.R. China and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 57110, P.R. China |
| |
| Abstract: | Sulfide is one of the common water pollutants in aquaculture. To investigate the toxic effects of sulfide stress on shrimp haemocytes, total haemocyte count (THC), reactive oxygen species (ROS) production, NO production and apoptotic cell ratio were determined in haemocytes of the white shrimp Litopenaeus vannamei exposure to sulfide (0.5 and 2.0 mg/L) for 6, 12, 24 and 48 h. Results showed that THC decreased to 117.8×105 cells/mL which was 72.7% of the control group (p<0.05), reduction, increases of ROS production was 225.2% compared with that of the control (p<0.05), and apoptotic cell ratio significantly increased to 7.42 % (p<0.05) were observed in shrimp exposed to 0.5 mg/L sulfide for 48 h., and 2.0 mg/L sulfide for 6 h, respectively. After 6 h exposure to 2.0 mg/L sulfide, THC significantly reduced (p<0.05), and ROS production and apoptotic ratio significantly increased (p<0.05). These results indicated that sulfide exposure induced superfluous production of ROS and caused oxidative stress, and then induced haemocyte apoptosis and caused subsequent THC reduction. This process may be an important mechanism for decline in cellular immunity of shrimp caused by sulfide stress. With the increase of sulfide concentration, its the cytotoxic effects of sulfide increased. NO production increased in shrimp exposed to 2.0 mg/L sulfide for 12 and 48 h. We speculate that NO may be a key factor and play a vital roleact as a signal factor in defense regulation against sulfide stress. |
| |
| Keywords: | sulfide Litopenaeus vannamei haemocyte apoptosis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《水生态学杂志》浏览原始摘要信息 |
| 点击此处可从《水生态学杂志》下载免费的PDF全文 |
|
