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| 响应面法优化嗜盐紫色硫细菌283-1培养基 | |
| 引用本文： | 崔小华,杨素萍,姚鹏.响应面法优化嗜盐紫色硫细菌283-1培养基[J].山西农业大学学报(自然科学版),2012,32(1):37-41. |
| 作者姓名： | 崔小华 杨素萍 姚鹏 |
| 作者单位： | 1. 山西大学生命科学学院,山西太原,030006 2. 华侨大学生物工程与技术系,福建厦门,361021 3. 山西中医学院中西医结合临床医学系,山西太原,030024 |
| 基金项目： | 国家自然科学基金(31070054);福建省自然科学基金(2010J01209) |
| 摘 要： | 为了提高紫色硫细菌283-1的生物量,对其培养基组分进行优化。采用单因子试验、Plackett-Burman设计法、最陡爬坡试验及中心组合设计响应面分析,研究了乙酸钠、碳酸氢钠、氯化铵、硫化钠、硫酸镁和无机盐6个因子对菌株283-1生物量的影响。结果表明,乙酸钠和硫化钠是影响该菌株生物量的主要因素,并获得了最佳培养基配方:乙酸钠1.45g·L-1,碳酸氢钠0.75g·L-1,氯化铵4.5g·L-1,硫酸镁0.5g·L-1,硫化钠1.05g·L-1,氯化钙0.19g·L-1,氯化钾0.34g·L-1,氯化钠50g·L-1,VB1220μg·L-1,无机盐溶液1mL·L-1。优化后菌株283-1的生物量提高了6倍,结果与理论预测值相近。 |
| 关 键 词： | 紫色硫细菌 生物量 响应面分析 培养基组分 |
Optimization of Medium Composition of the Halophilic Purple Sulfur Bacterium 283-1 Using the Response Surface Methodology |
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| Cui Xiaohua , Yang Suping , Yao Peng.Optimization of Medium Composition of the Halophilic Purple Sulfur Bacterium 283-1 Using the Response Surface Methodology[J].Journal of Shanxi Agricultural University,2012,32(1):37-41. | |
| Authors: | Cui Xiaohua Yang Suping Yao Peng |
| Institution: | 1.School of Life Science,Shanxi University,Taiyuan Shanxi 030006,China;2.Department of Bioengineering and Biotechnology,Huaqiao University,Xiamen Fujian 361021,China;3.Department of Clinical Chinese and Western Medicine,Shanxi University of Traditional Chinese Medicine,Taiyuan Shanxi 030024,China) |
| Abstract: | In order to improve the biomass,medium composition of the strain 283-1 of purple sulfur Bacterium was optimized.Effects of the six factors CH3COONa,NaHCO3,NH4Cl,Na2S,MgSO4 and inorganic salt on the biomass were evaluated by using single-factor test,Plackett-Burman design,the steepest ascent experiment and central composite design combined with response surface analysis in this work.The results showed that sodium acetate and sodium sulfide were the most significant factors and the optimal media were as following: CH3COONa 1.45 g·L-1,NaHCO3 0.75 g·L-1,NH4Cl 4.5 g·L-1,MgSO4 0.5 g·L-1,Na2S 1.05 g·L-1,CaCl2 0.19 g·L-1,KCl 0.34 g·L-1,NaCl 50 g·L-1,VB12 20 μg·L-1 and inorganic salt 1 mL·L-1.With the optimized media,the biomass improved 6 times compared with the control and the value was in agreetment with the predicted. |
| Keywords: | Purple sulfur bacteria Biomass Response surface analysis Medium composition |
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