| 稳定性同位素探针技术在土壤功能微生物原位鉴定的应用 |
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| 引用本文： | 蔺中,孙礼勇,陈昊,孙迎韬,白婧,李永涛.稳定性同位素探针技术在土壤功能微生物原位鉴定的应用[J].农业环境保护,2012(1):1-6. |
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| 作者姓名： | 蔺中 孙礼勇 陈昊 孙迎韬 白婧 李永涛 |
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| 作者单位： | 华南农业大学资源环境学院,广州510642 |
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| 基金项目： | 国家自然科学基金（40871152 41171210） |
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| 摘 要： | 新近发展起来的稳定性同位素探针技术（SIP）与分子生物学方法结合,能够定向发掘复杂环境中参与特定生态过程的微生物资源,是土壤功能微生物原位鉴定的有效手段,具有广阔的应用前景。其原理是环境样品中的功能微生物代谢同化同位素标记的底物,通过对其生物标志物（即DNA、RNA、PLFA等）进行提取、分离、鉴定和比对分析,以此获取介导土壤物质转化和循环过程的功能微生物的直接信息。本文在分别介绍SIP技术在土壤功能微生物原位鉴定过程中的各种前处理方法、生物标志物选择及后续鉴定方法的基础上,综述了SIP技术在研究驱动土壤有机污染物生物降解、碳氮循环等过程的功能微生物原位鉴定的应用进展,展望了SIP技术在土壤微生物基因组学方面的应用前景。
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| 关 键 词： | 土壤 稳定性同位素探针技术 原位鉴定 功能微生物 |
Application of Stable Isotope Probing on In-situ Identification of Soil Functional Microorganism |
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| LIN Zhong,SUN Li-yong,CHEN Hao,SUN Ying-tao,BAI Jing,LI Yong-tao.Application of Stable Isotope Probing on In-situ Identification of Soil Functional Microorganism[J].Agro-Environmental Protection,2012(1):1-6. |
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| Authors: | LIN Zhong SUN Li-yong CHEN Hao SUN Ying-tao BAI Jing LI Yong-tao |
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| Institution: | (College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | Utilization of stable isotope probing（SIP） coupled with molecular biology method,can explore microorganisms source responsible for specific process in natural environment,and has a broad application prospects for soil functional microorganism in situ identification.The principle of the SIP technique is as follows.Functional microorganisms in soil samples,that metabolize the SIP-labeled substrates and assimilate SIP-labeled biomarkers,e.g.,DNA,RNA and PLFA,can be identified by extraction,separation and analysis of these SIP-labeled biomarkers,and then information of soil functional microorganisms responsible for transformation and cyclic process of substrate in soil can be obtained directly as a result.With introduction of pre-treatment technology,SIP-labeled biomarker selection,and subsequent microorganisms＇ identification method,this study illustrates the research progress of SIP technique in in-situ identification of functional microorganisms involving in the soil organic pollutants degradation,carbon and nitrogen cycle.It will provide a comprehensive reference for the application and exploration of SIP-based in-situ identification of soil microbial genomics in future. |
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| Keywords: | soil stable isotope probing in-situ identification functional microorganism |
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