| 猪圆环病毒2型河北株的全基因组克隆与系统进化分析 |
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| 引用本文： | 王光祥,郑海学,刘湘涛,尚佑军,常艳燕,靳野.猪圆环病毒2型河北株的全基因组克隆与系统进化分析[J].动物医学进展,2008,29(10). |
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| 作者姓名： | 王光祥 郑海学 刘湘涛 尚佑军 常艳燕 靳野 |
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| 作者单位： | 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,国家口蹄疫参考实验室,甘肃,兰州,730046 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 应用PCR方法从疑似断奶仔猪多系统衰竭综合征（PMWS）的死亡仔猪组织病料中扩增出PCV-2全基因组（1767bp），将此基因片段克隆入pMD20-T载体，筛选获得重组质粒pMD-PCV2并对其进行序列测定，然后对全基因组进行同源性和遗传进化分析。结果表明，PCV-2分离毒株与参考株的全基N组同源性介于94％～98．4％之间，ORF1的同源性介于97．0％～98．8％，ORF2的同源性略低，介于91．9％～98．4％之间。该毒株与荷兰株、广西株和上海株等在一个进化分支上。本研究有助于监测PCV-2的疫源和进化关系，为进一步深入研究病毒基因功能奠定一定基础。
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| 关 键 词： | 猪圆环病毒2型 基因组 克隆 序列分析 |
Cloning and Evolution Relationship Analysis of Complete Genomes of Type 2 Porcine circovirus Isolated from Hebei Province |
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| WANG Guang-xiang,ZHENG Hai-xue,LIU Xiang-tao,SHANG You-jun,CHANG Yan-yan,JIN Ye.Cloning and Evolution Relationship Analysis of Complete Genomes of Type 2 Porcine circovirus Isolated from Hebei Province[J].Progress In Veterinary Medicine,2008,29(10). |
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| Authors: | WANG Guang-xiang ZHENG Hai-xue LIU Xiang-tao SHANG You-jun CHANG Yan-yan JIN Ye |
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| Institution: | National Foot-and-Mouth Disease Reference Laboratory;Key Laboratory of Animal Virology of the Ministry of Agriculture;State Key Laboratory of Veterinary Etiological Biology;Lanzhou Veterinary Research Institute;CAAS;Lanzhou;Gansu;730046;China |
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| Abstract: | The full-length nucleotide sequence of PCV-2 was amplified by PCR from the tissues of clinical pigs with postweaning multisystemic wasting syndrome.The fragment amplified was cloned into pMD 20-T vector and the recombinant plasmid was sequenced and determined.Then,the homology and evolutional relationship of PCV-2 genome and reference strain sequences were analyzed.The results of homology analysis showed that genome of Hebei isolate shared 94.0%-98.4% homology with reference sequence and the ORF1 exhibited ... |
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| Keywords: | type 2 Porcine circovirus genome cloning sequence analysis |
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