| 大豆7S球蛋白(α+β)-亚基缺失型种质的α-与β-亚基基因的测序与分析 |
| |
| 引用本文： | 刘珊珊,孟凡立,刁桂珠,王志坤,葛玉君,郑天慧,姜自芹,曾蕊,李文滨.大豆7S球蛋白(α+β)-亚基缺失型种质的α-与β-亚基基因的测序与分析[J].中国农业科学,2009,42(2):419-424. |
| |
| 作者姓名： | 刘珊珊 孟凡立 刁桂珠 王志坤 葛玉君 郑天慧 姜自芹 曾蕊 李文滨 |
| |
| 作者单位： | 东北农业大学农学院大豆研究所/国家教育部大豆生物学重点实验室,哈尔滨,150030 |
| |
| 基金项目： | 哈尔滨市科技攻关计划，东北农业大学引进人才项目，博士后研究人员落户黑龙江科研启动资助项目，第四十一批中国博士后科学基金，国家高技术研究发展计划(863计划)，国家科技支撑计划 |
| |
| 摘 要： | 【目的】明确大豆7S球蛋白（α+β）-亚基双缺失特性是否是由于α-与β-亚基基因的缺失或变异引起的。【方法】聚丙烯酰胺凝胶电泳(SDS-PAGE)分析和α-与β-亚基基因的PCR扩增、克隆、测序和序列比较。【结果】该种质α-亚基基因特异性引物的PCR扩增结果与对照基本一致，β-亚基基因的则与对照不同。α-亚基基因扩增片段与对照的同源性较高，为90.9%, 二者的区别主要存在于扩增片段的5′-端;β-亚基基因间的同源性较低，仅为53.1%。另外，在该材料β-亚基基因扩增片段的两端，检测到一对短的反向重复序列。【结论】该种质α-与β-亚基同时缺失的特性不是由α-与β-亚基基因的缺失引起的;α-与β-亚基基因的扩增序列与对照存在一定的差异，基因序列间的差异是否为导致α-与β-亚基缺失的直接原因，尚需进一步研究确认。
|
| 关 键 词： | 大豆 7S球蛋白 α-与β-亚基 稀有亚基组合 DNA测序 |
| 收稿时间： | 2008-1-28 |
Sequence Analysis of α- and β-Subunit Genes in a Novel Soybean Germplasm Characterized by Absence of α- and β-Subunit of 7S Globulin |
| |
| LIU Shan-shan,MENG Fan-li,DIAO Gui-zhu,WANG Zhi-kun,GE Yu-jun,ZHENG Tian-hui,JIANG Zi-qin,ZENG Rui,LI Wen-bin.Sequence Analysis of α- and β-Subunit Genes in a Novel Soybean Germplasm Characterized by Absence of α- and β-Subunit of 7S Globulin[J].Scientia Agricultura Sinica,2009,42(2):419-424. |
| |
| Authors: | LIU Shan-shan MENG Fan-li DIAO Gui-zhu WANG Zhi-kun GE Yu-jun ZHENG Tian-hui JIANG Zi-qin ZENG Rui LI Wen-bin |
| |
| Institution: | Soybean Research Institute of Northeast Agricultural University Ministry of Education Key Laborabory of Soybean Biology
|
| |
| Abstract: | 【Objective】 The objective of this study was to elucidate whether the α- and β- null character was result from the absence or variance of α- and β-subunit genes. 【Method】 SDS-PAGE technique, PCR amplification, cloning and sequencing methods were used. 【Result】 With specific primers for α- subunit, the PCR band pattern between (α+β)-null type and normal type was identical, but the PCR band pattern of β-subunit gene was unique. For PCR amplified DNA fragment of the α-subunit gene, a high degree of nucleotide homology (90.9%) exists between the (α+β)-null type and normal type. Sequenced difference mainly exists in 5′-flanking. While, for β-subunit gene, a low degree of nucleotide homology (53.1%) was exists. Furthermore, a short inverted sequence was detected in PCR amplified DNA fragment of the β-subunit gene.【Conclusion】The deficiency character of α- and β-subunit of (α+β)-null type is not related to the deletion of a chromosome segment with the genes encoding α- and β-subunit of soybean 7S globulin. Differences exist in both α- and β-subunit gene sequence between (α+β)-null type and normal type soybean. Further studies to confirm whether the differences in gene sequence are main reason that result in the absence of α- and β-subunit are necessary. |
| |
| Keywords: | soybean 7S globulin α- and β- subunit novel subunit combination DNA sequencing |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
