| 土壤微生物群落结构研究方法进展 |
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| 引用本文： | 张瑞福,崔中利,李顺鹏.土壤微生物群落结构研究方法进展[J].土壤,2004,36(5):476-480,515. |
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| 作者姓名： | 张瑞福 崔中利 李顺鹏 |
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| 作者单位： | 南京农业大学农业部农业环境微生物工程重点开放实验室,南京,210095 |
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| 基金项目： | 国家高技术研究计划资助项目(2001.AA214121和2001.AA246081)。 |
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| 摘 要： | 分离培养技术在土壤微生物的研究中有很大的局限性，因为土壤中的微生物大部分还处于不能被分离培养状态。随着微生物研究技术的发展尤其是分子生物学技术的发展，一系列不依赖于培养的技术在土壤微生物研究中得到广泛应用。本文介绍了生物标志物法、SCSU、DNA复性分析、DGGE、TGGE、ARDRA、T-RFLP、SSCP、PAPD和ERIC—PCR图谱分析等方法在土壤微生物群落结构研究中的应用。
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| 关 键 词： | 微生物群落结构 不依赖培养 研究方法 |
ADVANCE IN METHODS FOR RESEARCH ON SOIL MICROBIAL COMMUNITY STRUCTURE |
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| ZHANG Rui-fu,CUI Zhong-li and LI Shun-peng.ADVANCE IN METHODS FOR RESEARCH ON SOIL MICROBIAL COMMUNITY STRUCTURE[J].Soils,2004,36(5):476-480,515. |
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| Authors: | ZHANG Rui-fu CUI Zhong-li and LI Shun-peng |
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| Institution: | Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, Nanjing Agric Univ., Nanjing 210095,Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, Nanjing Agric Univ., Nanjing 210095 and Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, Nanjing Agric Univ., Nanjing 210095 |
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| Abstract: | Isolation and incubation as a means to study soil microbial communities has severe limitations, since the majority of microbes in the soil are unculturable. With the development of the molecular biology, a series of techniques which are independent of culturing are being widely used in the research of soil microbial communities. In this paper, techniques, such as Biomarker, SCSU, DNA renaturation experiment, DGGE, TGGE, ARDRA, T-RFLP, SSCP, PAPD and ERIC-PCR, commonly used in this field were introduced. |
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| Keywords: | Microbial community Independent of culturing Research methods |
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