High frequency of M307a mutation at FUT1 locus, causing resistance to oedema disease, in an autochtonous polish pig breed, the zlotnicka spotted |
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| Authors: | By J Klukowska B Urbaniak M
wito&#x;ski |
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| Institution: | By J. Klukowska,B. Urbaniak,M. ?witoński |
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| Abstract: | Oedema disease and post-weaning diarrhoea (enteric colibacillosis) occur in pigs aged 4–12 weeks, and are responsible for considerable economic losses (B ertschinger et al. 1992). The typical clinical symptoms of oedema disease are neurological signs such as ataxia, convulsions and paralysis. At post-mortem examination, oedema is typically present at characteristic sites such as eyelids and forehead, stomach wall and mesocolon. The occurrence of these illnesses in the whole population is quite high, about 15%, and the mortality among the sick animals is about 90% (N ielsen 1986). The disease is associated with the colonization of the small intestine with toxigenic Escherichia coli strains of a limited number of serotypes: O138, O139, O141, O147, O157, O16, O108 (B ertschinger et al. 1990; W ittig et al. 1994; R ippinger et al. 1995). It was revealed that E. coli strain 107/86 (serotype: O139:K12), forms fimbriae which adhere in vitro to cells isolated from small intestine epithelium (B ertschinger et al. 1990). Genetic studies revealed that susceptibility to colonization by an Escherichia coli strain with fimbriae F18 is controlled by a dominant allele, and the resistance to colonization by a recessive allele (B ertschinger et al. 1993). The gene locus for E. coli F18 receptor (ECF18R) has been mapped to the porcine chromosome 6 (V& ouml ; geli et al. 1996). Following this finding, M eijerink et al. (1997) have cloned the α(1,2)-fucosyltransferase genes FUT1, FUT2 and the pseudogene FUT2P. Sequencing of the FUT1 gene in swine resistant to adhesion by F18 fimbriated E. coli revealed a transition (G → A) at 307 bp position (M307). Thus, the M307A allele was considered as a good marker for the selection of resistant animals (M eijerink et al. 1997; V& ouml ; geli et al. 1997). The main purpose of this paper was the recognition of the mutation in four Polish pig breeds, namely Polish Large White, Polish Landrace, Zlotnicka White and autochtonous Zlotnicka Spotted. |
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