| 不同大豆品种籽粒维生素E含量积累比较分析 |
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| 引用本文： | 罗健,冯雷,李冬梅,韩英鹏,赵雪,李文滨,姜振峰.不同大豆品种籽粒维生素E含量积累比较分析[J].大豆科学,2017,36(2). |
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| 作者姓名： | 罗健 冯雷 李冬梅 韩英鹏 赵雪 李文滨 姜振峰 |
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| 作者单位： | 东北农业大学大豆生物学教育部重点实验室/农业部北方大豆生物学与遗传育种区域重点实验室,黑龙江哈尔滨,150030 |
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| 基金项目： | 国家自然基金，国家“十二五”科技支撑计划，国家现代农业产业技术体系，黑龙江省教育厅科学研究项目 |
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| 摘 要： | 采用高效液相色谱技术法(HPLC)以东北三省及内蒙古大豆产区的180份种质资源为研究材料,检测其籽粒总维生素E含量及各组分含量,进而分析维生素E总含量及各组分含量间的相关性,选用代表性大豆品种在不同生育时期维生素E积累动态进行分析。结果表明:大豆种质资源籽粒维生素E积累是从R5期开始,初期积累量呈上升趋势,R6期积累量开始大幅度减少,但从R6至R8期积累量又呈现大幅度上升趋势,在R8期积累量趋于稳定。经筛选合丰50和L-21为高维生素E含量品种,黑秣食豆和茶秣食豆为低维生素E含量品种,代表性大豆品种籽粒维生素E含量差异主要体现在R6~R7时期,该时期最终决定代表性大豆品种最终维生素E含量,这为研究高维生素E大豆新品种选育提供了理论基础,同时为高维生素E相关基因位点和候选基因的挖掘奠定了表型鉴定基础。
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| 关 键 词： | 大豆 维生素E 生育酚 高效液相色谱 |
Comparative Analysis of Vitamin E Content Accumulation in Different Soybean Varieties |
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| LUO Jian,FENG Lei,LI Dong-mei,HAN Ying-peng,ZHAO Xue,LI Wen-bin,JIANG Zhen-feng.Comparative Analysis of Vitamin E Content Accumulation in Different Soybean Varieties[J].Soybean Science,2017,36(2). |
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| Authors: | LUO Jian FENG Lei LI Dong-mei HAN Ying-peng ZHAO Xue LI Wen-bin JIANG Zhen-feng |
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| Abstract: | In this study,high performance liquid chromatography (HPLC) was used to study the content of total vitamin E and the content of each component in 180 soybean germplasm resources which from the three provinces of Northeast China and Inner Mongolia.The content of total vitamin E and the content of each component were analyzed.The dynamic analysis of vitamin E accumulation in representative soybean cultivars at different growth stages was analyzed.The results showed that the accumulation of Vitamia E began to increase from the beginning of R5,the accumulation at R6 began to decrease,but the accumulation showed a significant upward trend from R6 to R8,The accumulation trends to be stable at R8.Hefeng 50 and L-21 were high content of vitamin E cultivars,and the content of vitamin E in the Heimoshidou and Chamoshidou were low content of vitamin E cultivars.The difference of vitamin E content in grains was mainly reflected in R6-R7 period,which ultimately determined the final vitamin E content,which provided a theoretical basis for the research of high vitamin E soybean variety breeding and also laid the basis of the high Vitamin E related gene site and candidate genes for phenotypic identification. |
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| Keywords: | Soybean Vitamin E Tocopherol High performance liquid chromatography |
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