| 工厂化组培香蕉分化芽中病毒的检测 |
| |
| 引用本文： | 饶雪琴,张曙光,高乔婉.工厂化组培香蕉分化芽中病毒的检测[J].华南农业大学学报,2005,26(1):64-66. |
| |
| 作者姓名： | 饶雪琴 张曙光 高乔婉 |
| |
| 作者单位： | 华南农业大学,资源环境学院,广东,广州,510642 |
| |
| 摘 要： | 采用间接ELISA法和PCR方法对组织培养的香蕉分化芽中黄瓜花叶病毒(CMV)、香蕉线条病毒(BSV)和香蕉束顶病毒(BBTV)分别进行了检测，用间接ELISA法抽检香蕉分化芽中的CMV，带毒率为2．5％左右；PCR法检测香蕉分化芽中：BSV和BBTV、40个送检样品中带BSV的为12．5％，22个送检样品中未检测到带BBTV的香蕉分化芽。
|
| 关 键 词： | 香蕉 组培 工厂化 分化 束顶病 黄瓜花叶病毒 组织培养 CMV 送检 间接ELISA法 |
| 文章编号： | 1001-411X(2005)01-0064-03 |
Detecting viruses in banana differentiated shoots from tissue culture |
| |
| RAO Xue-qin,ZHANG Shu-guang,GAO Qiao-wan.Detecting viruses in banana differentiated shoots from tissue culture[J].Journal of South China Agricultural University,2005,26(1):64-66. |
| |
| Authors: | RAO Xue-qin ZHANG Shu-guang GAO Qiao-wan |
| |
| Abstract: | Indirect ELISA and PCR methods were used to detect the existence of cucumber mosaic virus (CMV), banana streak virus (BSV) and banana bunchy top virus (BBTV) respectively in cultured differentiated banana shoots, the results showed that the rate of CMV-carriers was about 2.5% by indirect ELISA, the rate of BSV-carriers was 12.5% of 40 samples by PCR, and no BBTV-carrier was found in 22 samples by PCR. |
| |
| Keywords: | cucumber mosaic virus banana streak virus banana bunchy top virus indirect ELISA PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《华南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《华南农业大学学报》下载免费的PDF全文 |
