| 杉木DNA的简便提取方法 |
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| 引用本文： | 尤勇,洪菊生.杉木DNA的简便提取方法[J].林业科学研究,1998,11(1):111-113. |
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| 作者姓名： | 尤勇 洪菊生 |
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| 作者单位： | 中国林业科学研究院林业研究所 |
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| 摘 要： | 杉木ＤＮＡ的简便提取方法＊尤勇洪菊生关键词杉木针叶ＤＮＡ巯基乙醇次生物质杉木（Ｃｕｎｎｉｎｇｈａｍｉａｌａｎｃｅｏｌａｔａ（Ｌａｍｂ）Ｈｏｏｋ．）是我国的特有树种，其分子生物学研究目前在国内外开展的较少，其近缘种的研究也较少。为了开展杉木分子生物学的...
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| 关 键 词： | 杉木 针叶 DNA 巯基乙醇 次生物质 提取 |
| 收稿时间： | 9/5/1997 12:00:00 AM |
A Simple Procedure for Extraction and Purification DNA in Chinese fir |
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| You Yong and Hong Jusheng.A Simple Procedure for Extraction and Purification DNA in Chinese fir[J].Forest Research,1998,11(1):111-113. |
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| Authors: | You Yong and Hong Jusheng |
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| Institution: | You Yong Hong Jusheng |
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| Abstract: | A simple, quick and reliable procedure was developed for the extraction and purification of DNA from the leaves of Chinese fir. Specifically designed to overcome the trouble caused by the very high concentration of pigments,polysaccharides and other contaminants in the leaves. In this procedure,the application of extraction buffer with high concentration of mercaptoethanol (2%)was proved to be successful in removing pigments,ethanol precipitation of resuspended nucleic acids from 3 mol/L NaAC and revealed to be powerful in removing polysaccharides,and PVP 360 was used to remove polyphenols. The resulted DNA with this method was of high molecular weight (more than 50 kb) and was completely digestible with restriction endonucleases and amplifiable in PCR,they are well suited to RAPD,RFLP analysis and other molecular marker systems. This procedure can also be used to extract DNA from other gymnosperms,for example: Taiwania fiousiana,Metasequoia glyptostroboides,Cryptomeria fortunei etc. |
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| Keywords: | Chinese fir needle DNA mercaptoethanol secondary metabolite |
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