| 竹子的离体培养研究 |
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| 引用本文： | 王敬文,蒋晶.竹子的离体培养研究[J].林业科学研究,1998,11(6):640-646. |
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| 作者姓名： | 王敬文 蒋晶 |
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| 作者单位： | 中国林业科学研究院亚热带林业研究所 |
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| 摘 要： | 近２０ａ来已对２０个属７０余种竹子进行离体培养研究，以侧芽，顶芽，成熟胚作外植体诱导愈伤组织，由愈伤组织制备悬浮细胞进行细胞悬浮培养，由悬浮细胞制备原生质体进行原生质体培养。竹子愈伤组织经不定芽途径或体细胞胚发生途径再生完整植株。通过芽尖培养增殖新生芽进行竹微繁殖，并获得脱病毒种苗。以芽为外植体增殖的新芽或组织再生苗经继代培养诱导竹试管开花结实。
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| 关 键 词： | 竹子 愈伤组织 悬浮细胞 原生质体 离体培养 |
| 收稿时间： | 4/7/1998 12:00:00 AM |
Study on Culture in vitro of Bamboo |
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| Wang Jingwen and Jiang Jing.Study on Culture in vitro of Bamboo[J].Forest Research,1998,11(6):640-646. |
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| Authors: | Wang Jingwen and Jiang Jing |
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| Institution: | The Research Institute of Subtropical Forestry, CAF Fuyang, Zhejiang 311400;The Research Institute of Subtropical Forestry, CAF Fuyang, Zhejiang 311400 |
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| Abstract: | species of bamboo in 20 genera were cultured in vitro in recent 20 years.Plant regeneration via somatic embryogenesis and adventitious shoot was achieved in callus cultures derived from explants excised axially buds,shoot apices or mature zygotic embryos.Liquid suspension culture cells of bamboo served as protoplasts donors,and high yields of viable protoplasts were obtained,and callus from protoplasts of suspension cultured bamboo cells were recovered. About 70 species of bamboo tested were successfully propagated in vitro . For nearly each species,multiple shoots were produced from axially buds on stem node segments cultured on MS medium containing BA. A few callus plant could be regenerated adventitiously from callus. In vitro flowering was induced on shoot developed from nodal explant taken from regenerated plants. The flowering of bamboo shoots of some species occurred in unison,but not that of other species. |
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| Keywords: | bamboo callus cell suspension protoplast micropropagation in vitro flowering |
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