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两株不同地区副猪嗜血杆菌的分离及分子生物学鉴定
引用本文:苗立中,李峰,吴忆春,付强,王艳,刘吉山,沈志强.两株不同地区副猪嗜血杆菌的分离及分子生物学鉴定[J].中国动物检疫,2009,26(5):47-48.
作者姓名:苗立中  李峰  吴忆春  付强  王艳  刘吉山  沈志强
作者单位:1. 山东省滨州畜牧兽医研究院,山东滨州,256618
2. 山东省滨州职业学院生物工程系,山东,滨州,256603
摘    要:本试验从浙江和河南部分地区的送检的疑似患多发浆膜炎与关节炎猪的病料中分离到2株疑似副猪嗜血杆菌,进行了细菌形态观察、培养特性和生化特性鉴定,根据已报道的副猪嗜血杆菌16SrRNA序列特异性引物进行PCR扩增,得到预期的822bp的基因片断,最终证实为副猪嗜血杆菌。

关 键 词:副猪嗜血杆菌  分离鉴定

Characterization of Haemophilus parasuis Isolated from Two Different Areas
Miao Lizhong,Li Feng,Wu Yichun,Fu Qiang,Wang Yan,Liu Jishan,Shen Zhiqiang.Characterization of Haemophilus parasuis Isolated from Two Different Areas[J].China Journal Of Animal Quarantine,2009,26(5):47-48.
Authors:Miao Lizhong  Li Feng  Wu Yichun  Fu Qiang  Wang Yan  Liu Jishan  Shen Zhiqiang
Institution:1 (Animal Science and Veterinary Medicine, Shandong, Binzhou, China, 256618) (Biological Engineering Department of Binzhou Vocational College, Shandong, Binzhou, China, 256603)
Abstract:Two Hps strains were isolated from suspected specimens collected from Zhejiang and Henan provinces.To evaluate the characteristics of the Hps strains,the phenotypic,biochemical and physiological analyses were performed,and the results showed the bactria were related to the family Pasteurel laceae.16S rRNA-based PCR confirmed the isolates and demonstrated that the bacteria are assigned to Hps.
Keywords:Haemophilus parasuis  isolation and identification
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