| 大丽轮枝菌蛋白激发子PevD1诱导棉花抗病性及作用机理 |
| |
| 引用本文： | 卜冰武,邱德文,曾洪梅,郭立华,袁京京,杨秀芬.大丽轮枝菌蛋白激发子PevD1诱导棉花抗病性及作用机理[J].植物病理学报,2014,44(3):254-264. |
| |
| 作者姓名： | 卜冰武 邱德文 曾洪梅 郭立华 袁京京 杨秀芬 |
| |
| 作者单位： | 中国农业科学院植物保护研究所/农业部作物有害生物综合治理重点实验室，北京 100081 |
| |
| 基金项目： | 国家高技术研究发展计划(“863”计划)(2011AA10A205) |
| |
| 摘 要： | PevD1是一种大丽轮枝菌(Verticillium dahliae)分泌蛋白，具有激发烟草过敏反应(HR)和系统获得性抗病(SAR)的功能。为明确蛋白PevD1诱导棉花抗病性及其作用机制，本文利用大肠杆菌表达、纯化的PevD1诱导棉苗植株，检测棉苗对大丽轮枝菌的抗性及免疫应答反应。结果表明，大肠杆菌表达的PevD1重组蛋白不是棉花品种“新陆早42号”的致萎因子，叶片注射8 μg/mL PevD1蛋白诱导3 d后根部接种大丽轮枝菌，15 d后PevD1处理组病害减轻率达35.04%。PevD1能诱导棉花叶片抗性早期信号分子H2O2产生和NO积累，维管束细胞壁加厚、木质素和酚类物质的积累。另外，PevD1处理能提高防御酶PAL、POD和PPO活性，提高棉花抗性基因和木质素合成相关基因PAL、C4H1、4CL的转录水平。说明PevD1通过激发棉花免疫系统而提高抗病性，该研究不仅为利用PevD1蛋白激发子控制棉花黄萎病提供了科学依据，同时也为阐明棉花与大丽轮枝菌互作机理提供了理论基础。
|
| 关 键 词： | 蛋白激发子PevD1 棉花黄萎病 大丽轮枝菌 诱导抗病性 抗病防御反应 |
| 收稿时间： | 2013-08-07 |
Induced resistance and mechanism of protein elicitor PevD1 against Verticillium dahliae in cotton |
| |
| BU Bing-wu,QIU De-wen,ZENG Hong-mei,GUO Li-hua,YUAN Jing-jing,YANG Xiu-fen.Induced resistance and mechanism of protein elicitor PevD1 against Verticillium dahliae in cotton[J].Acta Phytopathologica Sinica,2014,44(3):254-264. |
| |
| Authors: | BU Bing-wu QIU De-wen ZENG Hong-mei GUO Li-hua YUAN Jing-jing YANG Xiu-fen |
| |
| Institution: | Key Laboratory of Integrated Pest Management in Crop, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100081, China |
| |
| Abstract: | PevD1, a secrete elicitor from Verticillium dahliae, activated hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco. In order to investigate the induced resistance and mechanisms of PevD1 in cotton, the PevD1 was expressed in E. coli and assayed the cotton resistance against V. dahliae and immune responses. The results showed that purified recombinant protein PevD1 was not a phytotoxic factor in No.42 Xin-luzao cotton variety. Injection of PevD1 in leaves improved cotton resistance at a concentration as low as 8 μg/mL, the highest disease reduction was 35.04% at the 15th day post V. dahliae inoculation. This protein was able to systemically induce hydrogen peroxide and nitric oxide generation, vessel reinforcement, lignin and terpenoids deposition. PevD1 enhanced the expression of pathogenesis-related genes and lignin biosynthesis-related genes PAL, C4H1 and 4CL, and also increased enzyme activity of PAL, POD and PPO. The results demonstrated that the PevD1 protein triggered the plant immune responses and improved disease resistance in cotton. The research not only lay a theoretical foundation for cotton Verticillium wilt disease control but also provided a scientific basis for elucidating plant-elicitor interactions. |
| |
| Keywords: | protein elicitor PevD1 cotton Verticillium wilt Verticillium dahliae induced resistance defense response |
|
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
