| 芝麻枯萎病病原菌致病力室内鉴定方法 |
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| 引用本文： | 仇存璞,张海洋,常淑娴,魏利斌,苗红梅.芝麻枯萎病病原菌致病力室内鉴定方法[J].植物病理学报,2014,44(1):26-35. |
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| 作者姓名： | 仇存璞 张海洋 常淑娴 魏利斌 苗红梅 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室, 南京 210095; 河南省农业科学院河南省芝麻研究中心, 郑州 450002 |
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| 基金项目： | 国家现代农业(芝麻)产业技术体系建设专项资金(CARS-15); 国家科技支撑计划(2009BADA8B04); 公益性行业(农业)科研专项(nyhyzx07-015); 河南省科技厅“中原学者”项目(092101211100) |
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| 摘 要： | 芝麻枯萎病(Sesame Fusarium wilt, SFW)是由尖孢镰刀菌芝麻专化型(Fusarium oxysporum Schl. f. sp. sesami (Zap.), FOS)引起的一种土传真菌病害, 是世界芝麻生产上的主要病害之一。为测定FOS致病力, 本文选用郑芝98N09等4个芝麻品种, 在苗期对15个FOS菌株的致病力强弱进行了室内鉴定和评价。结果表明, 采用1×106个/mL分生孢子悬浮液与无菌蛭石和无菌土壤按V1∶V2∶V6比例混合接菌(即最终接菌浓度为1.4×105孢子/g土壤), 在接菌后第7 d幼苗开始出现枯萎病症状, 调查菌株致病性的最佳时间为接菌后第25 d~28 d;在供试15个FOS菌株中, 对4个品种均表现为强致病力的菌株有8个(DI>50), 均表现为弱致病力的菌株有5个(DI<20);不同芝麻品种对不同菌株的抗性有一定差异。该方法可应用于芝麻枯萎病病原菌致病性测定和芝麻种质抗枯萎病特性评价, 并为后续的机理研究提供了技术支持。
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| 关 键 词： | 芝麻 枯萎病 尖孢镰刀菌 致病力 鉴定 |
| 收稿时间： | 2013-01-20 |
Laboratory detecting method for pathogenicity of Fusarium oxysporum Schl. f. sp. sesami isolates |
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| QIU Cun-pu,ZHANG Hai-yang,CHANG Shu-xian,WEI Li-bin,MIAO Hong-mei.Laboratory detecting method for pathogenicity of Fusarium oxysporum Schl. f. sp. sesami isolates[J].Acta Phytopathologica Sinica,2014,44(1):26-35. |
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| Authors: | QIU Cun-pu ZHANG Hai-yang CHANG Shu-xian WEI Li-bin MIAO Hong-mei |
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| Institution: | National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China |
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| Abstract: | Sesame Fusarium wilt (Fusarium oxysporum f. sp. sesami, FOS) is an important disease in sesame production. In order to estabolish an effective evalutating method for FOS pathogenecity, 15 strains were tested on Zhengzhi 98N09 and other three cultivars of sesame at young seedling stage. The results indicated that the inoculated seedlings showed wilt symptom in seven days after inoculation with 1×106 microconidia/mL suspension mixed with aseptic vermiculite and soil under the ratio of V1∶V2∶V6 (i. e., the final concentration of 1.4×105 microconidia/g soil). The optimal date for investigating pathogenicity was the 25-28 th days after inoculation. Among the tested 15 FOS strains, eight strains showed high pathogenicity (DI>50)and five strains showed low or no pathogenicity (DI<20) on the four cultivars. The results also showed that different cultivars exhibited the various resistance levels to 15 strains. This technique could be applied for the evaluation of the FOS pathogenicity and sesame resistance to FOS, and give the technological supports for further exploration of these mechanisms. |
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| Keywords: | sesame Fusarium wilt Fusarium oxysporum pathogenicity identification |
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