| 反应温度对斜带髭鲷消化酶活力的影响 |
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| 引用本文： | 齐红莉,王睿睿,王晓梅,李琳,徐立坡.反应温度对斜带髭鲷消化酶活力的影响[J].安徽农业科学,2010,38(3):1293-1296. |
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| 作者姓名： | 齐红莉 王睿睿 王晓梅 李琳 徐立坡 |
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| 作者单位： | 1. 天津农学院水产科学系,天津市水产生态及养殖重点实验室,天津,300384
2. 国家海洋局大连环境监测中心,辽宁大连,116026 |
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| 基金项目： | 天津市教委创新项目(20060205);;天津农学院教改项目(08-C-01) |
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| 摘 要： | ［目的］研究不同反应温度对斜带髭鲷主要消化酶活性的影响。［方法］采用酶学分析方法研究了反应温度对斜带髭鲷消化系统6个部位蛋白酶、淀粉酶和脂肪酶活力的影响。［结果］胃、幽门垂、前肠、中肠、后肠、肝胰脏内，蛋白酶最适反应温度分别为40～45、40、35～40、40、40、40 ℃；淀粉酶的最适反应温度分别为40、30、30、30、40、30 ℃；脂肪酶最适反应温度分别为40、40、35～40、40、40、25 ℃。在最适反应温度下，斜带髭鲷不同消化器官内各消化酶的活力顺序为蛋白酶的活力是胃＞肝胰脏＞后肠＞前肠＞中肠＞幽门垂；淀粉酶的活力是前肠＞中肠＞后肠＞幽门垂＞肝胰脏＞胃；脂肪酶的活力是后肠＞中肠＞前肠＞幽门垂＞肝胰脏＞胃。［结论］在设定的反应温度范围内，斜带髭鲷各消化酶的活力均随反应温度的升高呈现先上升后降低的变化趋势，并存在器官特异性。
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| 关 键 词： | 斜带髭鲷 消化酶活力 反应温度 |
Effects of Reaction Temperature on the Activities of Digestive Enzymes in Hapalogeonys nitens Richardson |
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| QI Hong-li et al.Effects of Reaction Temperature on the Activities of Digestive Enzymes in Hapalogeonys nitens Richardson[J].Journal of Anhui Agricultural Sciences,2010,38(3):1293-1296. |
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| Authors: | QI Hong-li |
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| Institution: | Department of Fishery Science;Tianjin Agricultural University;Tianjin Key Laboratory of Aquatic Ecology and Aquaculture;Tianjin 300384 |
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| Abstract: | Objective] The purpose was to study the effects of different reaction temperature on the activity of main digestive enzymes in Hapalogeonys nitens Richardson. Method] Effects of reaction temperature on the activities of 3 digestive enzymes including protease, amylase and lipase in 6 organs of H. nitens were studied by means of enzymology analysis. Result] The optimum reaction temperature in stomach, pyloric caecas, foregut, mid-intestine, hindgut and liver were 40-45, 40, 35-40, 40, 40 and 40 ℃ resp. for protease, 40, 30, 30, 30, 40 and 30 ℃ for amylase, 40, 40, 35-40, 40, 40 and 25 ℃ for lipase. Under the optimum reaction temperature, the activities of digestive enzymes in different organs were in the order of stomach＞liver＞hindgut＞foregut＞mid-intestine＞pyloric caecas for protease, foregut＞mid-intestine＞hindgut＞pyloric caecas＞liver＞stomach for amylase, hindgut＞mid-intestine foregut＞pyloric caecas＞liver＞stomach for lipase. Conclusion] The activities of all digestive enzymes in H. nitens showed a trend of first increasing and then decreasing with the increment of reaction temperature in the designed reaction temperature ranges and had the organic specificity. |
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| Keywords: | Hapalogeonys nitens Richardson Activities of digestive enzymes Reaction temperature |
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