| 不同重悬细胞法在洗涤过程中对水牛外周血单个核细胞活率的影响 |
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| 引用本文： | 韩忠燕,张宝红,黄海愉,张为宇,黄维义,钟恒禄.不同重悬细胞法在洗涤过程中对水牛外周血单个核细胞活率的影响[J].中国畜牧兽医,2009,36(4):100-102. |
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| 作者姓名： | 韩忠燕 张宝红 黄海愉 张为宇 黄维义 钟恒禄 |
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| 作者单位： | （广西大学动物科学技术学院， 南宁 530005） |
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| 基金项目： | 广西自然科学基金，国家自然科学基金 |
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| 摘 要： | 为了探讨在离心洗涤水牛外周血单个核细胞（peripheral blood mononuclear cell, PBMC）过程中以移液器吹打法和晃动离心管法重悬细胞对其活率的影响。对经密度梯度离心后初步收集到的PBMC，分别用移液器吹打和晃动离心管2种方法重悬细胞，离心洗涤后对细胞活率进行计数。结果发现，移液器吹打法得到的细胞活率为（74.9±2.6）%，而晃动离心管法使细胞活率达到（98.5±0.5）%，差异极显著（P<0.01）。说明在洗涤PBMC时，晃动离心管法重悬细胞能大大提高细胞活率，可为那些对细胞活率要求高的试验提供一个较为有效的方法。
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| 关 键 词： | 水牛外周血单个核细胞 重悬细胞法 细胞活率 |
Influence of Buffalo PBMC Activity by Different Resuspension Methods During Washing Process |
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| HAN Zhong-yan,ZHANG Bao-hong,HUANG Hai-yu,ZHANG Wei-yu,HUANG Wei-yi,ZHONG Heng-lu.Influence of Buffalo PBMC Activity by Different Resuspension Methods During Washing Process[J].China Animal Husbandry & Veterinary Medicine,2009,36(4):100-102. |
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| Authors: | HAN Zhong-yan ZHANG Bao-hong HUANG Hai-yu ZHANG Wei-yu HUANG Wei-yi ZHONG Heng-lu |
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| Institution: | (College of Animal Science and Technology, Guangxi University, Nanning 530005, China) |
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| Abstract: | The peripheral blood mononuclear cell (PMBC) activity of buffalo was comported by different resuspension methods during washing process.The PBMC was separated by density gradient centrifugation，then cell was resuspended by transferpettor-spirting method ＆ centrifuge tube-sloshing method, when cell was washed three times the activity was evaluated. The cell activity by transferpettor-spirting method ＆ centrifuge tube-sloshing method was（74.9±2.6）% and（98.5±0.5）% respectively, the difference of cell activity was significant(P<0.01). The activity of PBMC was higher by centrifuge tube-sloshing, which could provide an useful method for further study that requiring higher cell activity. |
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| Keywords: | buffalo peripheral blood mononuclear cell resuspension methods cell activity |
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