| 烟草种子经离子注入后在TMV、CMV侵染时有关生理生化指标的变化 |
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| 引用本文： | 吐尔逊·伊不拉音,李茜,武宝山,王军,张石峰,曾宪贤,葛菊芬,郝微丽,刘炜.烟草种子经离子注入后在TMV、CMV侵染时有关生理生化指标的变化[J].种子,2001(5):5-7. |
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| 作者姓名： | 吐尔逊·伊不拉音 李茜 武宝山 王军 张石峰 曾宪贤 葛菊芬 郝微丽 刘炜 |
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| 作者单位： | 1. 新疆大学,乌鲁木齐,830008
2. 乌鲁木齐蔬菜研究所,830000 |
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| 基金项目： | 国家烟草专卖局资助研究项目 |
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| 摘 要： | 本文以离子注入的烟划种子所培育的烟株为试验材料，研究了人工接种花叶病毒（TMV）和黄瓜花叶病毒（CMV）后烟叶中有关生理生化指标的变化。结果表明：病毒浸染后烟草叶片电解质外渗率增大，但离子注入后烟草的电解质外渗率增幅小于对照；病毒侵染后烟草叶片的超氧化物歧化酶（SOD）、过氧化物酶（POD）、硝酸还原酶（NR）的活性及叶绿素含量均有所下降，但离子注入后烟草的上述酶活降幅较小；CAT活性在接毒后呈先低后高的变化，而对照在接毒后10天该酶活性高出离子注入烟草2倍多，随后剧降，到接毒25天时仅为离子注入处理烟株1／2。烟草种子经离子注入后，上述内源保护酶的活性在病毒浸染后能长时间的保持较高活性，这对烟草抗病性的提高十分重要。
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| 关 键 词： | 烟草种子 离子注入 电导率 叶绿素 内源保护酶活性 TMV CMV 抗病性 生理生化指标 |
Physiological and Biochemical Index Changes of Tobacco Leaf Infected with Mosaic Virus Whose seed Being Injected Ion |
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| Zhou Jiheng,Lin Guihua,Shang guan,Qiu biaoreng,Xiong Xiuan,Pan Long.Physiological and Biochemical Index Changes of Tobacco Leaf Infected with Mosaic Virus Whose seed Being Injected Ion[J].Seed,2001(5):5-7. |
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| Authors: | Zhou Jiheng Lin Guihua Shang guan Qiu biaoreng Xiong Xiuan Pan Long |
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| Institution: | Zhou Jiheng 1 Lin Guihua 2 Shang guan 2 Qiu biaoreng 2 Xiong Xiuan 1 Pan Long 1 |
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| Abstract: | Physiological and biochemical index changes of tobacco leaf being infected TMV and CMV were studied by testing tobacco strain whose seeds having been injected ion. The results were as follows: Being infected virus, the electrolyte exosmosis rate of tobacco leaf was increased , while the increase rate of the leaf being injected ion was less than that of the control. The activities of SOD?POD?NR and the chlorophyll content were decreased ,while the decreasing rates of the ion injected leaves were less than those of the controls. The CAT activities were decreasing at first, then began to increase . while the activities of the control was doubled 10 days after infected virus, then began to decrease markedly and was only half of that whose seed being injected ion. Tobacco seeds` being injected ion, endogenous protective enzyme aboveementioned were keeping higher activities for a long time after having been infected virus. It was so important to improve the tobacco resisting virus. |
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| Keywords: | Tobacco Ion injection Electric conductivity Chlorophyll Endogenous protective enzyme activity |
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