| 水稻Wx基因的遗传多态性及其与主要米质指标的相关性分析 |
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| 引用本文： | 万映秀,邓其明,王世全,刘明伟,周华强,李平.水稻Wx基因的遗传多态性及其与主要米质指标的相关性分析[J].中国水稻科学,2006,20(6):603-609. |
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| 作者姓名： | 万映秀 邓其明 王世全 刘明伟 周华强 李平 |
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| 作者单位： | 1.四川农业大学 水稻研究所， 四川 温江 611130； E mail:wanyingxiu@163.com;2.四川农业大学 西南作物基因资源与遗传改良教育部重点实验室， 四川 雅安 625014； *通讯联系人， E-mail:liping@cngk.com |
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| 基金项目： | 教育部长江学者奖励计划 |
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| 摘 要： | 利用RM190和484/W2R ACCⅠ标记分析了50个水稻非糯品种的Wx基因的等位性变异。其中，RM190揭示出7种(CT)n纯合变异类型和1种杂合类型，分别为 (CT)20、(CT)19、(CT)18、(CT)17、(CT)14、(CT)11、(CT)10和(CT)11/(CT)18，并以（CT）11和（CT）18两种类型为主；484/W2R ACCⅠ共揭示出2种纯合类型和1 种杂合类型，分别为G/G型、T/T型及G/T型；(CT)11大多为G/G型，(CT)18大多为T/T型，这两个标记可将供试材料划分为10种等位基因变异类型。进一步分析表明，RM190揭示的Wx基因多态性可以解释直链淀粉含量变异的59.3 %；484/W2R ACCⅠ揭示的Wx基因多态性可以分别解释直链淀粉含量及胶稠度变异的56.1%和24.6%；而两标记共同可解释直链淀粉含量变异的72.4%。还对这两个标记在育种实践中的应用前景进行了探讨。
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| 关 键 词： | 蜡质基因 微卫星标记 酶切扩增多态性序列标记 糊化温度 胶稠度 直链淀粉含量 水稻 多态性 |
| 文章编号： | 1001-7216（2006）06-0603-07 |
| 收稿时间： | 2006-01-20 |
| 修稿时间： | 2006-05-20 |
Genetic Polymorphism of Wx Gene and Its Correlation with Major Grain Quality Traits in Rice |
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| WAN Ying-xiu,DENG Qi-ming,WANG Shi-quan,LIU Ming-wei,ZHOU Hua-qiang,LI Ping.Genetic Polymorphism of Wx Gene and Its Correlation with Major Grain Quality Traits in Rice[J].Chinese Journal of Rice Science,2006,20(6):603-609. |
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| Authors: | WAN Ying-xiu DENG Qi-ming WANG Shi-quan LIU Ming-wei ZHOU Hua-qiang LI Ping |
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| Abstract: | An experiment was conducted to analyze the allelic variation of the Wx gene in 50 non-glutinous rice varieties(lines) using molecular markers RM190 for(CT)n single sequence repeat] and 484/W2R-ACCⅠ(for G/T SNP).A total of seven homozygous(CT)n-type variations,namely(CT)20,(CT)19,(CT)18,(CT)17,(CT)14,(CT)11 and(CT)10,and one heterozygous genotype(CT)11/(CT)18 were detected with RM190,of which(CT)11 and(CT)18 were predominant.Two homozygous(G/G and T/T genotypes) and one heterozygous G/T Wx genotype were detected using 484/W2R-ACCⅠ.Most of the materials with a(CT)11 of RM190 were G/G SNP of 484/W2R-ACCⅠ,while(CT)18 were T/T.The materials tested were grouped into 10 categories using the two markers together.Further analysis indicated that 59.3% variance of amylose content was attributed to the polymorphism of Wx gene resulted from RM190 marker,whereas 56.1% and 24.6% of the variance in amylose content and gel consistency were respectively attributed to the polymorphism of Wx gene resulted from 484/W2RACCⅠmarker.Furthermore,in the presence of SSR and CAPS markers together,72.4% of the variance in amylose content could be explained.The application prospects of the two markers in breeding were also discussed. |
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| Keywords: | waxy gene microsatellite cleaved amplified polymorphic sequence marker gelatinization temperature gelconsistency amylose content rice polymorphism |
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