| 鸡血清中三聚氰胺高效液相色谱检测方法的建立 |
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| 引用本文： | 包红朵,魏瑞成,黄思瑜,王冉.鸡血清中三聚氰胺高效液相色谱检测方法的建立[J].江苏农业学报,2009,25(6). |
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| 作者姓名： | 包红朵 魏瑞成 黄思瑜 王冉 |
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| 作者单位： | 江苏省农业科学院食品质量安全与检测研究所,农业部食品安全监控重点开放实验室,江苏省畜禽产品安全性研究重点实验室,江苏,南京,210014 |
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| 基金项目： | 国家"十一五"支撑计划，江苏省创新专项 |
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| 摘 要： | 以鸡血清为研究对象,考察了不同洗涤液和洗脱液浓度对三聚氰胺提取效率的影响.建立了鸡血清中三聚氰胺残留的简便高效液相色谱法,该方法采用 NH_2柱作为色谱分析柱,95%乙腈水溶液为流动相,样品经阳离子交换固相萃取柱净化、富集后,在紫外检测器214 ml处用高效液相色谱仪测定,保留时间在14～15 min.三聚氰胺浓度在0.05～5.0μg/ml范围内,相关系数(r)为0.999 9;在空白鸡血清样品中添加0.1 μg/ml、1.0 μg/ml和5.0 μg/ml浓度的回收率为78.05%-88.69%,相对标准偏差(RSD)为1.15%~8.33%;样品检测定量限为0.1μg/ml.
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| 关 键 词： | 高效液相色谱 三聚氰胺 鸡血清 固相萃取 |
Development of Analytical Method of Melamine Residues in Chicken Serum by HPLC |
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| BAO Hong-duo,WEI Rui-cheng,HUANG Si-yu,WANG Ran.Development of Analytical Method of Melamine Residues in Chicken Serum by HPLC[J].Jiangsu Journal of Agricultural Sciences,2009,25(6). |
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| Authors: | BAO Hong-duo WEI Rui-cheng HUANG Si-yu WANG Ran |
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| Abstract: | Chicken serum in this experiment was as the research object and the different washing liquid and the elu-ate concentration on the extraction efficiency of melamine were inspected. A simple high-performance liquid chromatography (HPLC) with NH_2 column and 95% aeetonitrile eluate to determine the melamine in chicken serum was described. Target analytes of samples were cleaned up and concentrated by HyperSep Retain-CX column selid-phase extraction (SPE). A perfect separation for melamine was achieved and retention time was 14-15 min at 214 nm. The standard curve was linear in the concentration range from 0. 05 μg/ml to 5.0 μg/ml, with correlation coefficient of 0. 999 9. Recoveries of melamine at fortified levels of 0. 1 μg/ml, 1.0 μg/Inl and 5.0 μg/ml ranged from 78. 05% to 88.69%, with relative standard devia-tion of 1.15% -8.33%. The quantification limit was 0. 1 μg/ml. |
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| Keywords: | high-performance liquid chromatography melamine chicken serum solid-phase extraction |
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