| 四重荧光定量PCR技术用于食品中4种肠道致病菌的检测 |
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| 引用本文： | 赵冉,;陈琼,;孔繁德,;徐淑菲,;杨涛,;许淑娟.四重荧光定量PCR技术用于食品中4种肠道致病菌的检测[J].经济动物学报,2014(3):161-167. |
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| 作者姓名： | 赵冉 ;陈琼 ;孔繁德 ;徐淑菲 ;杨涛 ;许淑娟 |
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| 作者单位： | [1]厦门市农产品质量安全检验测试中心,厦门361009; [2]厦门出入境检验检疫局.厦门361012,厦门361009; |
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| 基金项目： | 厦门市科技计划资助项目（3502220124011） |
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| 摘 要： | 用已建立的四重荧光PCR方法、传统国标或行标方法、MALDI Biotyper高通量微生物鉴定法(基质辅助激光解析电离飞行时间质谱法)对随机抽取的14份食品样品进行检测,比较3种检测方法的匹配性。并用四重荧光PCR对农贸市场、大型超市采集的10类食品共205份样品进行检测。旨在研究四重荧光PCR法在食品中检测沙门氏菌、弗氏枸橼酸杆菌、奇异变形杆菌和迟缓爱德华菌的应用及食品中这4种菌的污染情况。在205份食品样品中,受污染的食品有110份,污染率53.66%(110/205),同时检出两种及两种以上致病菌的样品有62份,占污染样品的56.36%(62/110)。迟缓爱德华菌大肠菌、弗氏枸橼酸杆菌、奇异变形杆菌、沙门氏菌的检出率分别为7.80%(16/205)、53.66%(110/205)、26.34%(54/205)、5.85%(12/205)。在这3种检测方法中,四重荧光PCR的特异性好、准确性高,操作简便省时,更适用于食品中4种致病菌的快速检测。抽检的食品样品中存在这4种菌的污染,尤以生禽畜肉和水产品污染严重,需加强对食品加工、流通环节的卫生监管和监测。
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| 关 键 词： | 多重荧光定量PCR 检测 食物 沙门氏菌 弗氏枸橼酸杆菌 奇异变形杆菌 迟缓爱德华菌 |
Detection of Four Pathogenic Entero-Bacteria in Food with Four Real-Time Fluorescent Quantitative PCR Technique |
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| Institution: | ZHAO Ran, CHEN Qiong , KONG Fan-de, XU Shu-fei, YANG Tao , XU Shu-juan( 1. Xiamen Agricultural Product Quality and Safety Testing Center, Xiamen 361009, China; 2. Xia- men Entry-Exit Inspection and Quarantine Bureau, Xiamen 361012, China) |
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| Abstract: | Tree methods including established Multiplex RTi PCR assay, traditional GB/SN stand- ards and MALDI-TOF-MS were used to detect Salmonella spp. , Citrobacterfreundii, Proteus mira- bills and EdwardsieUa tarda in 14 random food samples for comparing their results. Moreover, 205 food samples of 10 variety, which were random sampling from farm trade market and supermarket, were detected by four real-time fluorescent quantitative PCR. The method was used to research about the contamination situation of these four pathogenic bacterium in different food samples. The total de- tection rate of 205 samples was 53.66%（110/205）. The detection rate were 7.80% （16/205） for Edwardsiella tarda, 53.66% （ 110/205 ） for Citrobacter freundii, 26.34% （ 54/205 ） for Proteus mirabilis, 5.85 % （12/205） for Salmonella spp. 56.36% （62/110） samples were polluted by two orabove pathogenic bacterium. The multiplex RTi PCR assay was a highly specific, sensitive and rapid method, which could be used in the detection of the four food borne pathogenic bacteria at the same time. The key to prevent the event caused by food borne pathogenic bacteria or disease is to strengthen the food processing,health monitoring of the circulation. |
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| Keywords: | Four real-time fluorescent quantitative PCR detection food Salmonella spp Citrobacter freundii Proteus mirabilis Edwardsiella tarda |
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