| 不同猪种5-羟色胺受体基因的表达及其与肉质的相关性分析 |
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| 引用本文： | 王欢欢,;李庆海,;张雷,;楼立峰,;童训权,;章学东.不同猪种5-羟色胺受体基因的表达及其与肉质的相关性分析[J].养猪,2014(6):65-68. |
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| 作者姓名： | 王欢欢 ;李庆海 ;张雷 ;楼立峰 ;童训权 ;章学东 |
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| 作者单位： | [1]杭州市农业科学研究院,浙江杭州310024; [2]淳安花猪种业有限公司,浙江杭州311719 |
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| 基金项目： | 杭州市重大科技创新专项（20122512A03） |
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| 摘 要： | 为研究5-羟色胺（5-HT）受体基因在不同猪种的表达及其与肉质的关系,试验选取淳安花猪与杜长大三元猪各12头,同时饲喂,达上市体重时分别屠宰,测定胴体、肉质性状及肌肉、脂肪中的5-HT受体基因表达量。结果表明：淳安花猪、杜长大三元猪的平均屠宰体重分别为88.1、102.4kg;淳安花猪的板油率、背膘厚、肌内脂肪等指标均显著高于杜长大三元猪（P〈0.05）;屠宰率、眼肌面积杜长大三元猪显著高于淳安花猪（P〈0.05）。5-HT2A、5-HT7受体基因在2个猪种脂肪组织中的表达量均显著高于肌肉组织（P〈0.05）,杜长大三元猪的肌肉中5-HT2A、5-HT7表达量显著低于淳安花猪（P〈0.05）,而脂肪中表达量两猪种间差异不显著。5-HT2A、5-HT7受体基因表达量与屠宰肉质指标之间基本呈负相关,尤其是肌肉中的5-HT2A表达量,呈较强负相关（杜长大三元猪）或显著负相关（淳安花猪）。研究初步认为,5-HT受体基因在中外猪种之间存在表达差异,并与屠宰肉质性状具有相关性。
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| 关 键 词： | 5-HT2A 5-HT7 基因表达 肉质 |
Expression of 5-HT Receptor Gene and Its Association with Meat Quality in Different Pig Breeds |
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| Institution: | WANG Huanhuan, LI Qinghai, ZHANG Lei, LOU Lifeng, TONG Xunquan, ZHANG Xuedong (1.Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China; 2.Chun＇an Spotted Pig Breeding Co.,Ltd.,Hangzhou 311719,China) |
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| Abstract: | In order to investigate 5-HT receptor genes expression in different breeds and the relationship between meat quality and gene expression,we selected twelve Chun＆#39;an spotted pigs and twelve Duroc×Landrace× Yorksire crossbred pigs,uniform feeding and slaughtered at market weight.Carcass characteristics,meat quality traits and 5-HT receptor gene expression in longissimus muscle and subcutaneous fat were detected.The results showed that the average slaughter weight of Chun＆#39;an spotted pigs and Duroc×Landrace×Yorksire pigs were 88.1 kg and 102.4 kg respectively.Leaf fat rate,backfat thickness and intramuscular fat of Chun＆#39;an spotted pigs were significantly higher than Duroc×Landrace×Yorksire pigs （P〈0.05）.Slaughter rate,longissimus doris area of Duroc× Landrace×Yorksire pigs were significantly higher than Chun＆#39;an spotted pigs（P〈0.05）.5-HT2A,5-HT7 receptor gene expression levels of subcutaneous fat were significantly higher than longissimus muscle in two breeds （P〈0.05）;gene expression in longissimus muscle of Duroc ×Landrace ×Yorksire pigs was significantly lower than Chun＆#39;an spotted pigs （P〈0.05）,where as the difference of gene expression in subcutaneous fat was not significant.Gene expression of 5-HT2A and 5-HT7 showed negative correlation with carcass and meat quality traits.The study indicated that the expression of 5-HT receptor gene was different in Chinese and foreign pig breeds.Meanwhile, the expression levels had correlation with carcass and meat quality traits. |
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| Keywords: | 5-HT2A 5-HT7 gene expression meat quality |
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