| 基于Erns基因的猪瘟病毒一步法RT-PCR快速检测方法的建立和应用 |
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| 引用本文： | 于新友,;李天芝,;王金良,;李峰,;沈志强.基于Erns基因的猪瘟病毒一步法RT-PCR快速检测方法的建立和应用[J].养猪,2014(6):93-95. |
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| 作者姓名： | 于新友 ;李天芝 ;王金良 ;李峰 ;沈志强 |
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| 作者单位： | [1]山东绿都生物科技有限公司,山东滨州256600; [2]山东省滨州畜牧兽医研究院,山东滨州256600 |
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| 基金项目： | 山东省现代农业产业技术体系生猪产业创新团队项目（SDAIT-06-011-14 ） |
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| 摘 要： | 根据猪瘟病毒Erns基因保守序列设计了1对引物,建立了检测猪瘟病毒一步法反转录-聚合酶链（RT-PCR）方法,并对其特异性、敏感性进行了研究。该一步法RT-PCR对CSFV扩增结果为阳性,对照毒株扩增结果均为阴性;对猪瘟病毒检测的灵敏性为10 pg总RNA量。以上结果表明,该一步法RT-PCR特异性强、敏感性高、简便、快速,可用于猪瘟的早期确诊和病毒鉴定。
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| 关 键 词： | 猪瘟病毒 一步法RT-PCR 检测 |
Establishment and Application of a One-step RT-PCR Assay for Detection of CSFV on Erns Gene |
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| Institution: | YU Xinyou, LI Tianzhi, Wang Jinliang, Li Feng, SHEN Zhiqiang (1.Shandong Lvdu Bio-Industry Co.,Ltd.,Binzhou 256600,China; 2.Animal Science and Veterinary Medicine Academy,Binzhou 256600,China) |
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| Abstract: | A one-step RT-PCR assay for detection of CSFV was established using a pair of primers based on the Erns gene of classical swine fever virus （ CSFV ） . and the specificity , sensitivity were studied . This method specifically amplify a fragment from CSFV, but not from control virus. with a detection limit of 10 pg RNA of CSFV . Therefore the established one-step RT-PCR technique provided a sensitive , specific , fast and reliablemethod for diagnosis and epizootic study of the CSFV. |
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| Keywords: | classical swine fever virus （CSFV） one-step RT-PCR detection |
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