| 水稻颖壳退化突变体degraded hull 2 (dh2)的遗传分析与基因定位 |
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| 引用本文： | 郭爽,李云峰,任德勇,张天泉,何光华.水稻颖壳退化突变体degraded hull 2 (dh2)的遗传分析与基因定位[J].作物学报,2013,39(6):1039-1044. |
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| 作者姓名： | 郭爽 李云峰 任德勇 张天泉 何光华 |
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| 作者单位： | 西南大学水稻研究所 / 转基因植物与安全控制重庆市重点实验室，重庆 400716 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目，国家自然科学基金项目，中央高校基本科研业务费专项资金 |
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| 摘 要： | 鉴定和克隆水稻花器官突变体新基因，对了解水稻花器官发育的分子遗传机制和分子信号调控途径有着重要的作用。本研究报道了1个水稻颖壳异常突变体，来源于EMS (ethyl methane sulfonate)处理的缙恢10号(Oryza sativa)诱变群体，暂被命名为degraded hull 2 (dh2)。表型分析发现突变体小花第一轮内稃或外稃横向细胞数目减少，导致内稃或外稃变窄而不能正常勾合，从而呈现开裂现象，其内三轮花器官均无明显变化。遗传分析表明该突变性状受1个隐性单基因控制。利用群体分离分析法(bulked segregation analysis, BSA)，将DH2基因定位在第3染色体的IND-5和IND-14之间，遗传距离分别为0.99 cM和1.49 cM。该研究结果为DH2基因的图位克隆奠定了基础，对水稻花发育生物学研究具有重要的意义。
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| 关 键 词： | 水稻 degraded hull 2 (dh2) 遗传分析 基因定位 |
| 收稿时间： | 2012-10-31 |
Genetic Analysis and Gene Mapping of a degraded hull 2 (dh2) Mutant in Rice (Oryza sativa) |
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| GUO Shuang
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LI Yun-Feng
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REN De-Yong
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ZHANG Tian-Quan
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HE Guang-Hua.Genetic Analysis and Gene Mapping of a degraded hull 2 (dh2) Mutant in Rice (Oryza sativa)[J].Acta Agronomica Sinica,2013,39(6):1039-1044. |
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| Authors: | GUO Shuang LI Yun-Feng REN De-Yong ZHANG Tian-Quan HE Guang-Hua |
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| Institution: | Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China |
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| Abstract: | The identification and cloning of novel mutant genes of floral organ in rice play an important role in understanding the molecular genetic mechanisms and molecular signal pathways regulating floral organ development. A rice mutant, degraded hull 2(dh2), which was derived from ethylmethane sulfonate (EMS)-treated Jinhui 10 (Oryza sativa), exhibited defects in hull development. The dh2 floretsdisplayed open hull in whorl 1, however, the rest floral parts in other three whorls had no obvious change. Further analysis indicated that the number of transverse cells decreased, making the lemma or palea narrow, and causing the hull open. The genetic analysis revealed that the dh2 trait is controlled by a single recessive gene. Using the BSA method, the DH2 gene was finally mapped between IND-5 and IND-14 on chromosome 3 with geneticdistances of 0.99 cM and 1.49 cM, respectively. These results are useful for the map-based cloning of DH2 gene, and very important in the studies of floral development biology. |
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| Keywords: | Rice (Oryza sativa) degraded hull 2 (dh2) Genetic analysis Gene mapping |
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