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| 赣南脐橙绿霉病菌对常用杀菌剂抗性监测 | |
| 引用本文： | 王明爽,孙颖,陈国庆,方贻文,夏长秀,李红叶.赣南脐橙绿霉病菌对常用杀菌剂抗性监测[J].植物病理学报,2013,43(5):532-540. |
| 作者姓名： | 王明爽 孙颖 陈国庆 方贻文 夏长秀 李红叶 |
| 作者单位： | 1、浙江大学生物技术研究所,杭州 310029; 2、赣州市脐橙研究所,赣州 404000 |
| 基金项目： | 国家现代农业(柑橘)产业技术体系专项经费，公益性行业(农业)科研专项 |
| 摘 要： | 本文研究了来自赣南7个县的柑橘绿霉病菌(Penicillium digitatum)种群对该地区常用杀菌剂抑霉唑、咪鲜胺、甲基硫菌灵和百可得的抗性频率、抗性水平和对抑霉唑的抗性分子机制。结果表明:病菌对抑霉唑和咪鲜胺存在基本一致的抗性;2011和2012年病菌种群对抑霉唑和咪鲜胺的抗性频率分别为82%和90%,平均抗性倍数为51.5倍,抗性分子机制均属于IMZ-R3,即CYP51B基因启动子区发生199 bp插入的突变;病菌种群对甲基硫菌灵的抗性频率分别为82%和91%;病菌种群对百可得均表现敏感。本研究为采后柑橘病害防治药剂选择提供了科学的依据。 |
| 关 键 词： | 赣南脐橙 绿霉菌 杀菌剂抗性 抗性分子机制 |
Resistance monitoring of Penicillium digitatum from Gannan navel orange to commonly used fungicides |
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| WANG Ming-shuang , SUN Ying , CHEN Guo-qing , FANG Yi-wen , XIA Chang-xiu , LI Hong-ye.Resistance monitoring of Penicillium digitatum from Gannan navel orange to commonly used fungicides[J].Acta Phytopathologica Sinica,2013,43(5):532-540. | |
| Authors: | WANG Ming-shuang SUN Ying CHEN Guo-qing FANG Yi-wen XIA Chang-xiu LI Hong-ye |
| Institution: | 1、 Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China; 2、 Navel Orange Research Institute of Ganzhou, Ganzhou 404000, China |
| Abstract: | The frequency and level of resistance of Penicillium digitatum population from 7 counties of Gannan, Jiangxi Province to commonly used fungicide imazalil, prochloraz, thiophanate-methyl and iminoctadine tris, as well as the resistant molecular mechanism to imazalil were studied. The results showed that the consistent resistance to imazalil and prochloraz was presented in P. digitatum. The resistant frequencies of P. digitatum to imazalil were 82% and 90% in 2011 and 2012, respectively. The average resistant factor was 51.5. The resistance molecular mechanism of P. digitatum to imazalil from Gannan belonged to IMZ-R3, which was conferred by an insertion mutation of 199 bp in the promoter region of CYP51B gene. The resistant frequencies of P. digitatum to thiophanate-methyl were 82% and 91% in 2011 and 2012, respectively. However, all strains tested in the study were sensitive to fungicide iminoctadine tris. The study provided a scientific basis for the choice of fungicides in the post-harvest disease control of citrus.Key words:Gannan navel orange; Penicillium digitatum; fungicide resistance; molecular mechanism of fungicide resistance |
| Keywords: | Gannan navel orange Penicillium digitatum fungicide resistance molecular mechanism of fungicide resistance |
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