| 壳聚糖纯化黄芩黄酮工艺研究 |
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| 引用本文： | 毕红梅,朱登超,李曼,黄雅君,兰小兵,张伟,吴丹丹,周艳丽,梁英.壳聚糖纯化黄芩黄酮工艺研究[J].中兽医医药杂志,2013,32(3):56-58. |
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| 作者姓名： | 毕红梅 朱登超 李曼 黄雅君 兰小兵 张伟 吴丹丹 周艳丽 梁英 |
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| 作者单位： | 1. 黑龙江八一农垦大学,黑龙江大庆,163319
2. 黑龙江八一农垦大学,黑龙江大庆 163319;黑龙江省农垦宝泉岭管理局安监局,黑龙江宝泉岭154211 |
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| 基金项目： | 黑龙江省垦区科研项目，黑龙江八一农垦大学大学生创新性实验计划项目 |
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| 摘 要： | 目的：确定壳聚糖对黄芩黄酮浸提液纯化的优化工艺条件。方法：通过正交试验设计考察壳聚糖添加量、料液温度和pH值对黄芩黄酮浸提液纯化效果。结论：壳聚糖对黄芩黄酮浸提液具有较好的纯化作用,最佳工艺条件为A3B2C2,即药液温度50℃、壳聚糖加入量为2%、pH值为5。
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| 关 键 词： | 黄芩 黄酮类化合物 壳聚糖 纯化工艺 正交设计 |
Study on purifying techonology of flavonoids in Radix Scutellariae with chitosan |
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| BI Hong-mei,ZHU Deng-chao,LI Man,HUANG Ya-jun,LAN Xiao-bing,ZHANG Wei,WU Dan-dan,ZHOU Yan-li,LIANG Ying.Study on purifying techonology of flavonoids in Radix Scutellariae with chitosan[J].Journal of Traditional Chinese Veterinary Medicine,2013,32(3):56-58. |
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| Authors: | BI Hong-mei ZHU Deng-chao LI Man HUANG Ya-jun LAN Xiao-bing ZHANG Wei WU Dan-dan ZHOU Yan-li LIANG Ying |
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| Institution: | 1.Heilongjiang Bayi Agricultural University,Daqing Heilongjiang 163319;2.Safety Supervision Bureau,Baoquanling Bureau of Reclamation in Heilongjiang Province,Baoquanling Heilongjiang 154211,China) |
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| Abstract: | Objective：The optimal purifying technology of flavonoids from crude extractives of Radix Scutellariae with chitosan was studied.Motheds： The orthogonal test was adopted to explore the purification effects of chitosan concention,temperature and pH value on flavonoids,protein,polysaccharide and optical density.Results：Chitosan has better purification effect on flavonoids from Radix Scutellariae,and the optimum technical parameters was A3B2C2,i.e.chitosan concentration was 2%,temperature was 50℃,and pH value was 5.0. |
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| Keywords: | Radix Scutellariae flavonoids chitosan purifying technology orthogonal design |
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