| 两个水稻D2基因等位突变体的表型分析及分子鉴定 |
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| 引用本文： | 秦雁玲,尹亮,赵金凤,孙伟,赵庆雷,袁守江,朱文银,郭宝太,李学勇.两个水稻D2基因等位突变体的表型分析及分子鉴定[J].作物学报,2013,39(6):1013-1020. |
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| 作者姓名： | 秦雁玲 尹亮 赵金凤 孙伟 赵庆雷 袁守江 朱文银 郭宝太 李学勇 |
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| 作者单位： | 1 青岛农业大学生命科学学院，山东青岛266109；2 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程，北京100081；3 山东省水稻研究所，山东济南250100 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)项目，国家自然科学基金项目 |
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| 摘 要： | 通过EMS诱变粳稻品种日本晴，筛选到2个矮秆突变体s1-46和s1-96。突变体主要表现为株高降低，分别为野生型的44.7%和55.9%，且叶片直立，穗粒数减少，籽粒变短。暗处理时，野生型的中胚轴伸长，但突变体的不伸长，说明突变性状与油菜素内酯(BR)相关。外源活性BR处理后，突变体与野生型的叶夹角均变大，根长均变短，表明突变基因与BR的生物合成相关。遗传分析表明，该突变性状由1对隐性基因控制。通过与籼稻品种Dular杂交构建F2群体，将该基因定位在第1染色体40.9 kb范围内。测序表明，该基因与参与BR生物合成的D2基因等位，其中s1-46第305位的氨基酸由脯氨酸突变成亮氨酸，而s1-96第370位的甘氨酸突变成谷氨酸。
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| 关 键 词： | 水稻 矮秆突变体 油菜素内酯 精细定位 |
| 收稿时间： | 2012-11-13 |
Phenotypic Analysis and Molecular Characterization of Two Allelic Mutants of D2 Gene in Rice |
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| IN Yan-Ling,YIN Liang,ZHAO Jin-Feng,SUN Wei,ZHAO Qing-Lei,YUAN Shou-Jiang,ZHU Wen-Yin,GUO Bao-Tai,LI Xue-Yong.Phenotypic Analysis and Molecular Characterization of Two Allelic Mutants of D2 Gene in Rice[J].Acta Agronomica Sinica,2013,39(6):1013-1020. |
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| Authors: | IN Yan-Ling YIN Liang ZHAO Jin-Feng SUN Wei ZHAO Qing-Lei YUAN Shou-Jiang ZHU Wen-Yin GUO Bao-Tai LI Xue-Yong |
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| Institution: | 1.College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China;2.National Key Facility for Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;3.Shandong Rice Research Institute, Jinan 250100, China |
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| Abstract: | Two dwarf mutants s1-46 and s1-96 were isolated from an EMS mutated japonica cultivar Nipponbare. The plant height of the two mutants was 44.7% and 55.9% of that of the wild-type plant, respectively. The mutants also exhibited erect leaves, reduced grain number,,and shortened grains. When grown in complete darkness, the mesocotyl elongated in the wild-type plant, but not in the mutants, indicating that s1-46 and s1-96 are brassinosteroids (BRs)-related mutants. Furthermore, treatment of seedling with bioactive BR showed that the leaf angle was increased and the root length was decreased in both the wild-type and mutant plants, suggesting that the mutated gene is involved in BR biosynthesis. Genetic analysis showed that the phenotypes of both mutants were caused by a recessive gene. Fine-mapping showed that this gene was located within a 40.9 kb region on chromosome 1. Sequence analysis revealed that the mutated gene was allelic to D2, encoding CYP90D2that is involved in BR biosynthesis. In s1-46 and s1-96, the encoded amino acids were changed from proline to leucine and from glycine to glutamic acid at the 305th and370thpositions of D2, respectively. |
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| Keywords: | Rice Dwarf mutant BR (brassinosteroids) Fine-mapping |
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