In vitro plantlet regeneration from Australian Red Cedar (Toona ciliata,Meliaceae) |
| |
| Authors: | Mroginski Erika Rey Hebe Y Mroginski Luis A |
| |
| Institution: | (1) Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste (IBONE), Corrientes, C.C. 209 - (3400), Argentina |
| |
| Abstract: | In vitro regeneration of complete plants from nodal single-bud segments of 2-year-old Australian Cedar (Toona ciliata) trees were obtained under defined nutritional and environmental conditions. Explants were dissected from plants obtained by germination of seeds and growth in pots in a greenhouse. The best medium for shoot regeneration was that of Murashige and Skoog at 1/4 strength with 3% sucrose (1/4 MS), supplemented with 0.1 mg/l IBA and 0.5 mg/l BAP. Rooting of regenerated shoots was observed in MS medium with 0.1 mg/l IBA. Using mature tree material was more difficult. Forced flushing was used to induce shoot development on branches of a 10-year-old tree. Nodal segments of these epicormic shoots formed shoots in vitro on 1/4 MS + 0.01 mg/l IBA + 5 mg/l BAP, but rooting was never observed. |
| |
| Keywords: | Micropropagation Nodal culture Plant regeneration Tissue culture |
| 本文献已被 SpringerLink 等数据库收录！ |
