| 聚乙二醇引发对盐胁迫下棉种萌发及生理特性的影响 |
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| 引用本文： | 肖爽,韩雨辰,号宇然,王晓蕾,刘连涛,孙红春,张永江,李存东.聚乙二醇引发对盐胁迫下棉种萌发及生理特性的影响[J].核农学报,2021,35(1):202-210. |
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| 作者姓名： | 肖爽 韩雨辰 号宇然 王晓蕾 刘连涛 孙红春 张永江 李存东 |
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| 作者单位： | 河北农业大学农学院/省部共建华北作物改良与调控国家重点实验室/河北省作物生长调控重点实验室,河北 保定 071001 |
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| 基金项目： | 国家自然科学基金(31871569);河北省产业技术体系(HBCT2018040201);国家重点研发计划(2018YFD0100306)。 |
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| 摘 要： | 为提高棉花在盐胁迫下的出苗率,争取全苗、壮苗,以棉花种子为试验材料,使用蒸馏水和5%、10%、15%聚乙二醇(PEG-6000)进行6、12、24 h引发,根据发芽势(GP)和发芽率(GR)筛选出最适引发条件。后续以未引发种子为对照,使用筛选出的最佳引发条件进行引发,分别于4个NaCl浓度(0、50、100、150 mmol·L-1)下进行萌发,研究种子引发对盐胁迫下棉种萌发和生理特性的影响。结果表明,使用5% PEG-6000引发12 h为最佳引发条件,并且在后续的NaCl胁迫下同样表现突出。在不同浓度的NaCl胁迫下,引发后种子的GP、GR、发芽指数(GI)和活力指数(Ⅵ)均较未引发出现了显著提高。对萌发7 d棉种形态特征的研究发现,下胚轴长度和根长在不同浓度的NaCl胁迫下分别提高了17.95%~30.37%和12.93%~74.96%。种子生理指标的测定结果表明,经过引发的棉种萌发7 d其根系活力提高了6.84%~39.32%;萌发2、4、6 d,种子超氧化物歧化酶(SOD)、过氧化物酶(POD)活性与未引发种子相比同样出现了较大幅度的提升;而种子内部的H2O2、丙二醛(MDA)含量却降低,说明PEG引发可以通过增强种子内部抗氧化酶活性和缓解膜脂过氧化程度来提高棉种的耐盐能力。本研究为提高棉花在盐胁迫下的出苗率提供了简单、有效的方法。
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| 关 键 词： | 棉花 萌发 聚乙二醇 种子引发 盐胁迫 |
| 收稿时间： | 2019-06-19 |
Effects of Polyethylene Glycol Priming on Germination and Physiological Characteristics of Cotton Seeds Under Salt Stress |
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| XIAO Shuang,HAN Yuchen,HAO Yuran,WANG Xiaolei,LIU Liantao,SUN Hongchun,ZHANG Yongjiang,LI Cundong.Effects of Polyethylene Glycol Priming on Germination and Physiological Characteristics of Cotton Seeds Under Salt Stress[J].Acta Agriculturae Nucleatae Sinica,2021,35(1):202-210. |
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| Authors: | XIAO Shuang HAN Yuchen HAO Yuran WANG Xiaolei LIU Liantao SUN Hongchun ZHANG Yongjiang LI Cundong |
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| Institution: | College of Agronomy, Hebei Agricultural University/State Key Laboratory of North China Improvement and Regulation/Key Laboratory of Crop Growth Regulation of Hebei Province, Baoding, Hebei 071001 |
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| Abstract: | In order to increasing the emergence rate of cotton under salt stress,strive for whole seedlings and strong seedlings,the cotton seeds were used as experiment materials primed by distilled water and 5%,10%,15%polyethylene glycol(PEG-6000)for 6,12,and 24 hours,respectively.The optimum primer and priming time were selected according to germination potential(GP)and germination rate(GR).To explore the effects of seed priming on germination and physiological characteristics of cotton seeds under salt stress,the seeds primed by the optimum initiator were germinated at four NaCl concentrations(0,50,100,150 mmol·L-1),and the non-primed seeds were used as control.The results showed that 5%PEG-6000 for 12 hours was the best priming condition,and it also showed prominent performance under subsequent NaCl stress.The seed germination potential(GP),germination rate(GR),germination index(GI)and vigor index(Ⅵ)were significantly higher than those without priming under different NaCl stresses.It was found that hypocotyl length and root length increased by 17.95%~30.37%and 12.93%~74.96%,respectively,under different salt stresses on 7 days of germination.The results of seed physiological indices showed that the root activity increased by 6.84%~39.32%after 7 days of seed germination,and the activities of superoxide dismutase(SOD)and peroxidase(POD)also increased significantly on 2,4 and 6 d of seed germination compared with that of non-primed seeds,while the contents of hydrogen peroxide(H2O2)and malondialdehyde(MDA)in seed decreased.These results indicated that PEG priming could increase the root activity,and the salt tolerance of cotton seeds was improved by enhancing the activity of antioxidant enzymes in seeds and alleviating the degree of membrane lipid peroxidation.This study provides a simple and effective method to improve the emergence rete of cotton under salt stress. |
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| Keywords: | cotton germination polyethylene glycol seed priming salt stress |
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