| 水雍菜浮床养殖模式下黄颡鱼肠道菌群结构分析 |
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| 引用本文： | 石焱,马徐发,吴志新,任雨薇,陈孝煊.水雍菜浮床养殖模式下黄颡鱼肠道菌群结构分析[J].华中农业大学学报,2016,35(2):69-76. |
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| 作者姓名： | 石焱 马徐发 吴志新 任雨薇 陈孝煊 |
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| 作者单位： | 华中农业大学水产学院/农业部淡水生物繁育重点实验室/淡水水产健康养殖湖北省协同创新中心,武汉,430070 |
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| 基金项目： | 国家科技支撑计划项目（2012BAD25B05）；国家自然科学基金项目（31472310） |
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| 摘 要： | 通过大规模养殖试验,研究人工浮床水培水雍菜对鱼塘养殖废水中氮磷的去除能力,并采用高通量测序方法研究养殖前后和不同处理组黄颡鱼肠道的微生物菌群结构,并分析了它们之间的相关性。结果表明:人工浮床水培水雍菜对鱼塘养殖废水中氨氮、总氮和总磷有良好的去除作用。养殖前后黄颡鱼肠道细菌多样性有明显差异;不同处理组之间的黄颡鱼肠道细菌种类多样性存在一定的差异,但优势菌群的组成并没有明显的结构差异,优势菌种的构成相似。在养殖前期黄颡鱼肠道中变形菌门(88.56%)占主要优势,末期对照组(无浮床)以厚壁菌门(48.4%)、梭杆菌门(32.52%)和变形菌门(14.56%)为主,试验组(有浮床)以变形菌门(36.72%)、厚壁菌门(21.89%)、梭杆菌门(17.48%)和拟杆菌门(11.48%)为主。
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| 关 键 词： | 黄颡鱼 肠道 水雍菜 人工浮床 菌群结构 高通量测序 |
| 收稿时间： | 2015/5/28 0:00:00 |
Intestinal microbial communities of yellow catfish (Pseudobagrus fulvidraco) in pond with Ipomoea aquatica forsk planted on artificial floating beds |
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| Abstract: | The removal capacity of nitrogen and phosphorus by Ipomoea aquatica Forsk planted on artificial floating beds were emphatically studied,and the microflora structure in the yellow catfish (Pseudobagrus fulvidraco) intestine were analyzed by using high-through sequencing technique.The results showed that ammonia nitrogen,total nitrogen and total phosphorus can be removed by the I. aquatica Forsk planted on artificial floating beds,with high removal efficiency.There were some differences in the intestinal bacteria diversity between the experimental (with artificial floating beds) and control (without artificial floating beds) groups.But the bacterial composition of different groups did not significantly vary from each other,and the predominant species were similar in all groups.At the beginning period,Proteobacteria was dominant (88.56%) in the yellow catfish intestine,and at the ending period,the dominant bacterial community in the control group were Firmicutes (48.4%),Fusobacteria (32.52%) and Proteobacteria (14.56%),whereas in the experimental group,the core microbiota composed of Proteobacteria (36.72%),Firmicutes (21.89%),Fusobacteria (17.48%) and Bacteroidetes (11.48%).This study provided a more comprehensive understanding that I. aquatica Forsk planted on artificial floating beds can change the flora structure of the yellow catfish intestine. |
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| Keywords: | yellow catfish (Pseudobagrus fulvidraco) intestine Ipomoea aquatica Forsk artificial floating beds microflora structure high-through sequencing technique |
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