Analysis of adventitious root formation in isolated stem explants of Rhododendron |
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| Authors: | RLM Pierik HHM Steegmans |
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| Institution: | Department of Horticulture, Agricultural University, Wageningen The Netherlands |
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| Abstract: | Factors affecting adventitious root formation were studied in isolated stem segments of three Rhododendron cultivars, ‘Catawbiense Album’, ‘Pink Pearl’ and ‘Van Weerden Poelman’, having different rooting ability. The experiments consisted of varying certain factors, while keeping the others constant.Root formation was always less in ‘Van Weerden Poelman’ than in the easy-to-root cultivars. Rooting usually occurred only in segments from young soft shoots. The length of the explants did not affect rhizogenesis. Removal of a strip of bark promoted adventitious root formation. Oxygen supply appeared to be essential since only inverted segments were capable of forming roots. Darkness was essential for rooting. The optimum temperature was 25°C. Root formation occurred only when a sugar was present in the medium. Rooting decreased with increasing osmotic pressure. Macroelements, boric acid and the pH of the medium did not play an essential role. Auxin was an absolute requirement for rooting, the kind and concentration depending on the cultivar used. Benzylamino purine at low concentrations increased root dry weight. GA3 and abscisic acid decreased rooting. |
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