Factors involved in increased protein synthesis in liver microsomes after administration of DDT |
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| Authors: | ID Cappon DM Nicholls |
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| Institution: | Department of Biology, York University, Downsview, Toronto, Ontario M3J 1P3, Canada |
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| Abstract: | Microsome fractions were prepared from liver homogenates of control rats and rats treated with DDT. The increased incorporation of 14C]phenylalanyl-tRNA into peptide when microsomes or ribosomes derived from DDT-treated rats were incubated with supernatant was due to factors in addition to increased endogenous mRNA. These factors were not related to the decreased activity of ribonuclease and increased activity of ribonuclease inhibitor, nor to the GTP and thiol sensitive inhibitor. The factors were partly removed from microsomes and completely removed from ribosomes by procedures using a 0.5 M KCl wash. Treatment of rats with polychlorinated biphenyls gave results similar to DDT. The 0.5 M KCl wash fraction from the control preparations contained factors inhibitory to protein synthesis in contrast to the wash fraction from the DDT preparations. |
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