| 纳米硒对齐口裂腹鱼生长、肌肉成分、血清生化及抗氧化指标的影响 |
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| 引用本文： | 李彦红,张飞飞,黄丽娟,石艳平,王依,廖茂雯,邬应龙.纳米硒对齐口裂腹鱼生长、肌肉成分、血清生化及抗氧化指标的影响[J].中国水产科学,2020,27(6):682-691. |
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| 作者姓名： | 李彦红 张飞飞 黄丽娟 石艳平 王依 廖茂雯 邬应龙 |
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| 作者单位： | 四川农业大学食品学院, 四川 雅安 625014 |
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| 基金项目： | 四川省科技厅重点研发项目（2019YFN0048）；四川农业大学“211”工程双支计划项目（2016）（03571650）. |
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| 摘 要： | 为研究纳米硒（nano-selenium，Nano-Se）对齐口裂腹鱼（）生长性能、肌肉成分、血清生化指标及抗氧化性能的影响，在基础饲料中分别添加0.0 mg/kg、0.2 mg/kg、0.4 mg/kg、0.8 mg/kg纳米硒，分别饲喂体重为（98.75±5.74）g的齐口裂腹鱼8周。结果表明，与对照组相比，不同添加剂量纳米硒均增加齐口裂腹鱼的特定生长率和肠体指数，降低饵料系数（<0.05）。另外，纳米硒各剂量组鱼体血清球蛋白、总胆固醇和甘油三酯含量，超氧化物歧化酶活性均显著升高，丙二醛和葡萄糖含量显著降低（<0.05）。日粮添加0.4 mg/kg和0.8 mg/kg的纳米硒时，血清谷胱甘肽过氧化物酶活性显著增加；日粮添加0.8 mg/kg的纳米硒，血清一氧化氮合酶活性和肌肉硒含量显著增加（<0.05）。综上，在促进齐口裂腹鱼的生长发育、改善肌肉品质以及提高血清抗氧化性能方面，3种剂量的纳米硒均产生了一定的影响，基于特定生长率的二次回归模型拟合，获得齐口裂腹鱼饲料中纳米硒最适添加量为0.52 mg/kg，综合考虑对生长性能和抗氧化能力的影响，推荐齐口裂腹鱼饲料中纳米硒的添加量为0.5 mg/kg。
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| 关 键 词： | 纳米硒 齐口裂腹鱼 生长性能 肌肉成分 抗氧化 |
Influence of dietary nano-selenium on growth, muscle composition, and serum biochemical and antioxidant indices of Schizothorax prenanti |
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| LI Yanhong,ZHANG Feifei,HUANG Lijuan,SHI Yanping,WANG Yi,LIAO Maowen,WU Yinglong.Influence of dietary nano-selenium on growth, muscle composition, and serum biochemical and antioxidant indices of Schizothorax prenanti[J].Journal of Fishery Sciences of China,2020,27(6):682-691. |
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| Authors: | LI Yanhong ZHANG Feifei HUANG Lijuan SHI Yanping WANG Yi LIAO Maowen WU Yinglong |
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| Institution: | College of Food Science, Sichuan Agricultural University, Ya''an 625014, China |
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| Abstract: | The present study aimed to determine the effects of dietary nano-selenium (0.0 mg/kg, 0.2 mg/kg, 0.4 mg/kg, 0.8 mg/kg) added to feed for eight weeks on growth, muscle composition, serum biochemical and antioxidant indices of individuals with similar body weight (98.75±5.74 g) were randomly assigned to four groups with three replicates per group and 30 fish per replicate. Fish were starved for 24 h before sampling, and body weight, length, and width were prepared for measurement of growth indicators. The head, kidneys, spleen, liver, mesonephros, and intestines were prepared for the immune organ index. Muscle was prepared for body composition analysis. Blood samples were collected from the caudal vein and were used directly to assay indicators in the serum of . The results were as follows. Compared with the control group (basal diet), specific growth rate (SGR) and intestinal weight index (IWI) were increased, and feed conversion ratio (FCR) was decreased, with the increasing of supplement of nano-selenium (<0.05). Weight gain rate (WGR) also increased when diets were supplemented with 0.2 mg/kg nano-selenium. The intestinal ratio (IR) of was significantly increased when 0.4 mg/kg and 0.8 mg/kg nano-selenium was added (<0.05). Additionally, the crude protein content of muscles was significantly increased when 0.4 mg/kg and 0.8 mg/kg nano-selenium was added (<0.05). Compared with the control group, the serum globulin (GLOB), triglyceride (TG), and cholesterol (TC) content of the fish were significantly increased when diets were supplemented with different doses of nano-selenium (<0.05). At the same time, the serum superoxide dismutase (SOD) activity of fish was significantly increased, and the serum malondialdehyde (MDA) and glucose (Glu) content of fish were significantly decreased (<0.05) in the Se supplemental group. The serum glutathione peroxidase (GSH-Px) activity of was significantly increased when 0.4 mg/kg and 0.8 mg/kg nano-selenium was added to the diet. Serum nitric oxide synthase (iNOS) activity and muscle selenium content was significantly increased when 0.8 mg/kg nano-selenium was added. Taken together, the results showed that nano-selenium had certain effects on the growth and development of the fish, and that it improved muscle quality and serum antioxidant performance. The optimal levels of dietary nano-selenium supplement in were estimated to be 0.52 mg/kg using quadratic regression analysis based on specific growth rate. Through comprehensive consideration of growth performance, antioxidant status, and lipid metabolism, the supplement level of 0.5 mg/kg nano-selenium is recommended in diets of . This study provides a new theoretical basis for the development of nutritional feed for . |
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| Keywords: | nano-selenium growth performance muscle composition antioxidant |
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