| 邻苯二甲酸二丁酯对厚壳贻贝抗氧化防疫系统的影响 |
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| 引用本文： | 许彩娜,曾姣,袁骐,隋延鸣,迟海,王翠华.邻苯二甲酸二丁酯对厚壳贻贝抗氧化防疫系统的影响[J].中国水产科学,2020,27(8):934-942. |
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| 作者姓名： | 许彩娜 曾姣 袁骐 隋延鸣 迟海 王翠华 |
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| 作者单位： | 1. 上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306;2. 中国水产科学研究院东海水产研究所, 农业农村部东海渔业资源开发利用重点实验室, 上海 200090 |
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| 基金项目： | 国家重点研发计划项目（2018YFD0900603）；国家自然科学基金青年科学基金项目（41706142）；中央级公益性科研院所基本科研业务费项目（1968-2017）. |
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| 摘 要： | 邻苯二甲酸二丁酯（dibutyl phthalate，DBP）作为增塑剂，已被中国列入优先控制的污染物名单，其对海洋生物的影响已引起人们的广泛关注。为研究邻苯二甲酸酯类化合物（phthalic acid esters，PAEs）对海洋贝类抗氧化防疫系统的影响，本文以厚壳贻贝（）为受试生物，研究了不同质量浓度DBP（0 mg/L、100 mg/L、200 mg/L、280 mg/L、360 mg/L）胁迫以及清水释放对其鳃和内脏团中抗氧化系统相关指标超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）活性以及丙二醛（malondialdehyde，MDA）含量变化的情况。结果表明：厚壳贻贝鳃和内脏团SOD、CAT活性以及MDA含量呈现不完全相同的变化规律。DBP胁迫阶段，鳃和内脏团中SOD活性基本呈先诱导后抑制再诱导的趋势，CAT活性则先抑制后诱导，MDA含量基本呈不断升高趋势；清水释放阶段，鳃和内脏团中SOD活性基本呈先抑制后诱导的趋势，CAT活性基本处于诱导状态，之后内脏团CAT活性被抑制，MDA含量处于先升高，之后内脏团MDA含量出现降低的趋势，浓度-效应和时间-效应较为明显。研究表明，在DBP胁迫下，厚壳贻贝鳃和内脏团产生了氧化损伤，可诱导机体产生脂质过氧化现象，且15 d清水释放期间不足以让厚壳贻贝恢复到正常水平。本研究旨在为海洋贝类的健康养殖和风险评价提供科学依据。
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| 关 键 词： | 邻苯二甲酸二丁酯 厚壳贻贝 抗氧化指标 内脏团 鳃 |
Effect of dibutyl phthalate on the antioxidant and epidemic prevention systems of Mytilus coruscus |
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| XU Cain,ZENG Jiao,YUAN Qi,SUI Yanming,CHI Hai,WANG Cuihua.Effect of dibutyl phthalate on the antioxidant and epidemic prevention systems of Mytilus coruscus[J].Journal of Fishery Sciences of China,2020,27(8):934-942. |
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| Authors: | XU Cain ZENG Jiao YUAN Qi SUI Yanming CHI Hai WANG Cuihua |
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| Institution: | 1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;2. Key Laboratory of East China Sea Fishery Resource Exploitation, Ministry of Agriculture and Rural Affairs;East China Sea Fisheries Research Institute, Chinese Academy o |
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| Abstract: | Dibutyl phthalate (DBP), a kind of plasticizer, had been included in the priority control pollutants list in China. The impact on marine organisms has caused widespread concern. At present, the research on the impact of DBP on marine organisms focuses on the growth, reproduction, and development of plankton, crustaceans, and fish. There are limited studies on the effects of antioxidant indicators in shellfish. Biochemical indicators such as antioxidants and disease resistance of organisms are important indicators of the degree of stress in response to pollutants and are widely used in ecotoxicology research. As one of the main varieties of seawater shellfish cultured in China, has a strong bioaccumulation in the marine environment and can be used as a biomonitoring species for environmental pollution. To study the changes of the antioxidant defense system, superoxide dismutase (SOD) and catalase (CAT) activity, and malondialdehyde (MDA) content in the gills and visceral mass of at five concentrations of DBP (0 mg/L, 100 mg/L, 200 mg/L, 280 mg/L, and 360 mg/L), with two stages of clear water flushes, were measured. This was to explore the effect of DBP stress on the biological indicators of and to determine whether the function of the body could be restored by the flushes of clear water. The experimental results show that the activities of SOD, CAT, and MDA content in gills and visceral mass of were not identical. Under DBP stress, SOD activity in gills and visceral mass was basically increased, then decreased, and then increased; CAT activity was decreased first and then increased; MDA content generally increased. When they were transferred to clean seawater, the activity of SOD in the gills and visceral mass of showed a trend of decreasing first and then increasing, and CAT activity was increased first. After CAT activity in the visceral mass decreased, and the MDA content in the gills increased, the visceral mass showed a trend of increasing CAT activity first and then decreasing, and the concentration-effect and time-effect were more obvious. This indicated that, under DBP stress, the body was induced to produce reactive oxygen free radicals to reduce the damage of free radicals to cells. With the prolongation of stress time and the increase in concentration, the activity of SOD and CAT in most concentration groups showed a trend of decreasing first and then increasing, which may be because of the inability of the antioxidant system in to remove excess oxygen free radicals in time. When the DBP stress exceeds the capacity of the body (which causes a certain amount of oxidative damage to the cells), SOD and CAT activity is inhibited, and the tendency to increase may be related to the metabolic consumption of DBP and the self-repairing regulation of . It can induce the body to perform lipid peroxidation, which will cause the increase in MDA content. This damage is difficult to return to normal with a release of clean seawater. Although SOD and CAT activity did not show complete synchronization, this phenomenon is consistent with the organism''s antioxidant enzyme response mechanism. In short, the change of antioxidant enzyme activity in marine organisms is a dynamic process, which may be affected by many factors such as environment, concentration, biological individual, and tissue differences. Therefore, it is necessary to comprehensively consider the response mechanism. The results of this paper evaluated the effect of DBP on from the perspective of oxidative damage, and provided reference data for the comprehensive understanding of the mechanism of DBP on marine organisms in the future, and provides a scientific basis for aquaculture health and risk assessment of marine shellfish. |
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| Keywords: | dibutyl phthalate antioxidant indexes visceral mass gill |
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