| 海水网箱养殖花鲈和日本黄姑鱼肠道细菌的多样性 |
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| 引用本文： | 张琛,王岩,郑侠飞,李云梦,任幸.海水网箱养殖花鲈和日本黄姑鱼肠道细菌的多样性[J].中国水产科学,2020,27(9):1113-1124. |
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| 作者姓名： | 张琛 王岩 郑侠飞 李云梦 任幸 |
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| 作者单位： | 1. 浙江大学海洋学院, 浙江 舟山 316021;2. 浙江大学动物科学学院, 浙江 杭州 310058;3. 上海实验动物研究中心, 上海 201203 |
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| 基金项目： | 国家自然科学基金面上项目（31772868）；浙江省重点研发项目（2018C02033）. |
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| 摘 要： | 利用Illumina MiSeq高通量测序技术分析了网箱养殖花鲈（）肠道细菌组成，同时分析了配合饲料和网箱中海水的细菌组成，以确定鱼类肠道细菌与环境之间的关系。花鲈投喂冰鲜鱼，日本黄姑鱼投喂配合饲料。从花鲈和日本黄姑鱼前肠、中肠、后肠、配合饲料和网箱内海水中共鉴定出886个细菌OTU（operational taxonomic unit），分别隶属8个门、40个属，其中从花鲈和日本黄姑鱼肠道中鉴定出的550个细菌OTUs分别隶属5个门、15个属。在门的水平上，厚壁菌门（Firmicutes）在两种鱼类肠道中均占优势；在属的水平上，梭菌属（）在日本黄姑鱼肠道中占优势。花鲈与日本黄姑鱼之间肠道细菌的相似性高于日本黄姑鱼肠道细菌与配合饲料中细菌的相似性，也高于花鲈或日本黄姑鱼肠道细菌与其养殖网箱内海水中细菌的相似性。日本黄姑鱼肠道与配合饲料中共有的细菌OTU数多于日本黄姑鱼肠道与网箱内海水中共有的细菌OTU数。综上所述得出结论：（1）花鲈和日本黄姑鱼肠道细菌群落分别独立于其养殖网箱内海水细菌群落，日本黄姑鱼肠道细菌群落独立于其配合饲料中的细菌群落；（2）日本黄姑鱼肠道细菌受配合饲料中细菌的影响大于受网箱内海水中细菌的影响。
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| 关 键 词： | 花鲈 日本黄姑鱼 肠道细菌 配合饲料 海水养殖 |
Bacterial diversity in the gut of Japanese seabass (Lateolabrax japonicus) and giant croaker (Nibea japonica) reared in seawater net pens |
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| ZHANG Chen,WANG Yan,ZHENG Xiafei,LI Yunmeng,REN Xing.Bacterial diversity in the gut of Japanese seabass (Lateolabrax japonicus) and giant croaker (Nibea japonica) reared in seawater net pens[J].Journal of Fishery Sciences of China,2020,27(9):1113-1124. |
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| Authors: | ZHANG Chen WANG Yan ZHENG Xiafei LI Yunmeng REN Xing |
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| Institution: | 1. Ocean College, Zhejiang University, Zhoushan 316021, China;2. College of Animal Sciences, Zhejiang University, Hangzhou 310058, China;3. Shanghai Laboratory Animal Research Center, Shanghai 201203, China |
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| Abstract: | The gut bacterial diversity of Japanese seabass () reared in nearshore net pens at Zhoushan were analyzed by high-throughput sequencing on an Illumina MiSeq platform. The Japanese seabass were fed raw fish, and the giant croaker were fed formulated feed. The bacterial composition of the formulated feed and the seawater in the net pens were also analyzed. A total of 886 operational taxonomic units (OTUs), belonging to 8 phyla and 40 genera, were identified from the fish guts, formulated feed, and seawater. A total of 550 OTUs, belonging to 5 phyla and 15 genera, were identified from the guts of the Japanese seabass and giant croaker. At the phylum level, Firmicutes dominated in the fish gut, while at the genus level, Bacillus dominated in giant croaker. Similarities in the bacterial communities were higher between guts of different fish species than between fish gut and the seawater in the net pen. This suggests that gut bacterial communities of Japanese seabass and giant croaker are independent of the seawater in the net pens. There were more common OTUs between the giant croaker gut and the formulated feed than between the giant croaker gut and the seawater, suggesting a greater impact of the feed on the intestinal bacterial community. |
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| Keywords: | , intestinal bacteria, formulated feed, seawater aquaculture |
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