| 2, 4-D浓度对胡萝卜悬浮细胞茄红素合成的影响 |
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| 引用本文： | 梁燕,王鸣,陈杭,陈大明.2, 4-D浓度对胡萝卜悬浮细胞茄红素合成的影响[J].西北农业学报,2002,11(2):46-49. |
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| 作者姓名： | 梁燕 王鸣 陈杭 陈大明 |
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| 作者单位： | 1. 西北农林科技大学,陕西杨凌,712100
2. 北京市蔬菜研究中心,北京板井,100081 |
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| 基金项目： | 国家自然科学基金项目(30070525)及北京市科技新星项目(9558101400). |
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| 摘 要： | 从茄红素含量，细胞总鲜重及茄红素总产量三方面，研究了培养基中，2，4－D浓度对胡萝卜悬浮培养细胞茄红素合成的影响。结果表明：2，4－D浓度在0．0－5．0mg/L，范围内，随着浓度升高单位鲜重细胞中茄红素含量增加，浓度为5.0mg/L时，含量最高，高于5.0mg/L时，茄红素含量急剧下降，2，4－D浓度在0.0-0.1mg/L范围内，浓度增加有利于细胞鲜重和茄红素总产量的提高，浓度高于0.1mg/L对两者都有抑制作用。
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| 关 键 词： | 2 4－D 悬浮细胞 合成 胡萝卜 茄红素 悬浮培养 施用浓度 |
| 文章编号： | 1004-1389(2002)02-0046-04 |
| 收稿时间： | 2002/4/21 0:00:00 |
| 修稿时间： | 2002年4月21日 |
The Effects of 2, 4-D Concentration on Lycopene Synthesis by Carrot Cells in Suspension |
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| LIANG Yan,WANG Ming,CHEN Hang and CHEN Daming.The Effects of 2, 4-D Concentration on Lycopene Synthesis by Carrot Cells in Suspension[J].Acta Agriculturae Boreali-occidentalis Sinica,2002,11(2):46-49. |
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| Authors: | LIANG Yan WANG Ming CHEN Hang and CHEN Daming |
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| Institution: | Northwest Sci Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;Northwest Sci Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;Beijing Vegetable Research Center, Banjing Beijing 100081, China;Beijing Vegetable Research Center, Banjing Beijing 100081, China |
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| Abstract: | The effects of 2,4-D concentrations on lycopene synthesis by carrot (Daucus carota L) cells in suspension were studied by measuring the changes of the lycopene content, total cell fresh weight and total lycopene yield with the 2,4-D concentrations in medium.The results showed that 2,4-D had great effect on lycopene synthesis. The lycopene contents increased with the concentrations increment from 0.0 mg/L to 5.0 mg/L, and declined deeply as the concentrations higher than 5.0 mg/L. The value of lycopene content at 5.0 mg/L was the highest of all treatments. The total cell fresh weight and total lycopene accumulation were all improved with the concentrations changing from 0.0 mg/L to 0.1 mg/L. |
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| Keywords: | 2 4-D Daucus carota 2 4-dichrolophenoxyacetic acid(2 4-D) Lycopene Suspension culture |
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