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毒害艾美耳球虫感染对雏鸡免疫器官IL-2表达及淋巴细胞增殖能力的影响
引用本文:赵伟,郑世民.毒害艾美耳球虫感染对雏鸡免疫器官IL-2表达及淋巴细胞增殖能力的影响[J].中国预防兽医学报,2011,33(4):309-312.
作者姓名:赵伟  郑世民
作者单位:1. 东北农业大学动物医学学院,黑龙江哈尔滨150030;佳木斯大学,黑龙江佳木斯154007
2. 东北农业大学动物医学学院,黑龙江哈尔滨,150030
摘    要:为了探讨毒害艾美耳球虫(E.necatrix)感染雏鸡后,其免疫器官白细胞介素(IL-2)和T、B淋巴细胞增殖能力的变化,本研究应用组织匀浆涂片和酸性α-醋酸萘酯酶(ANAE)染色及细胞培养技术和四甲基偶氮唑盐(MTT)测定法对E.necatrix感染雏鸡免疫器官的T淋巴细胞百分数、IL-2诱生活性、T和B淋巴细胞对C...

关 键 词:毒害艾美耳球虫  免疫器官  白细胞介素-2  T、B淋巴细胞数量及其增殖能力

Influence of Eimeria necatrix to proliferation of T and B lymphocytes and interleukin-2 induced activity in immune organs of chicks
ZHAO Wei,ZHENG Shi-min.Influence of Eimeria necatrix to proliferation of T and B lymphocytes and interleukin-2 induced activity in immune organs of chicks[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(4):309-312.
Authors:ZHAO Wei  ZHENG Shi-min
Institution:ZHAO Wei1,2,ZHENG Shi-min1* (1. College of Veterinary Medicine,Northeast Agricultural University,Harbin150030,China,2. Jiamusi University,Jiamusi 154007,China)
Abstract:Dynamic changes of percentage of T lymphocytes, IL-2 induced activity and proliferation of T and B lymphocytes to concanavalin A (ConA) or phorble myristate acetate (PMA) in immune organs of 14-day-old chicks infected with Eimeria necatrix were studied using acid nonspecific alpha-naphthyl acetate esterase (ANAE), cellular culture technique and MTT assay. These results showed that the IL-2 induced activity in thymus and spleen of chicks infected with E.necatrix was significantly increased compared with that...
Keywords:Eimeria necatrix  immune organ  interleukin 2  number and proliferation of T and B lymphocytes  
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