| 土著菌群与蚯蚓肠道菌群共享核心物种协同抵御灭蚁灵胁迫研究 |
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| 引用本文： | 朱国繁,应蓉蓉,邓绍坡,孔令雅,钱家忠,孙明明,朱瑜,叶茂.土著菌群与蚯蚓肠道菌群共享核心物种协同抵御灭蚁灵胁迫研究[J].土壤,2021,53(6):1250-1260. |
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| 作者姓名： | 朱国繁 应蓉蓉 邓绍坡 孔令雅 钱家忠 孙明明 朱瑜 叶茂 |
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| 作者单位： | 合肥工业大学,生态环境部南京环境科学研究所,生态环境部南京环境科学研究所,生态环境部南京环境科学研究所,合肥工业大学,南京农业大学,海南大学政治与公共管理学院,中国科学院南京土壤研究所 |
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| 基金项目： | 国家重点研发计划项目（2018FYC1803100）、国家自然科学基金面上项目（42077106）和中国科学院青年创新促进会项目（2018350） |
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| 摘 要： | 摘 要：灭蚁灵是一类高毒性的有机氯农药，当灭蚁灵通过直接或间接的人为途径进入到土壤生态环境系统后，会对生物体产生显著的毒害作用。土著菌群与蚯蚓肠道菌群都是对外源农药极其敏锐的感知受体，然而，它们对于抵御灭蚁灵毒害作用的微生物响应机制尚不清楚，值得探究。因而，本研究采集实际场地中不同梯度浓度（0~27.7 mg/kg）灭蚁灵污染土壤，设置蚯蚓土培实验，采用高通量测序技术，分析蚯蚓肠道菌群和土著菌群的结构和功能；通过MetagenomeSeq分析、LEfSe（Linear Discriminant Analysis Effect Size）分析和随机森林（Random Forests）分析以及网络分析，识别其核心类群。发现：1）灭蚁灵胁迫下蚯蚓肠道菌群相较之土著菌群的结构、组成受到的扰动更显著（p < 0.05）；2）不同梯度灭蚁灵胁迫下，蚯蚓肠道菌群和土著菌群组合存在稳定的核心物种，主要为气单胞菌属（Aeromonas）、黄杆菌属（Flavobacterium）、盖勒氏菌属（Gallerella）、微枝形杆菌属（Microvirga）、地杆菌属（Pedobacter）、亨氏菌（Ramlibacter）、Zavarzinella；3）这些核心种群在互作网络的平均度中心性、紧密中心性、特征向量中心性分别为136.72、0.44、0.52，均高于非核心种群的对应指标（91.52、0.42、0.33），表明核心种群具有更高的网络连通性，且此类核心种群具有碳氮转化和农药降解能力，说明蚯蚓肠道菌群和土著菌群可以通过共享核心物种与其它微生物联系紧密，具备发挥农药降解代谢的潜在功能，实现协同抵御土壤中灭蚁灵胁迫。本研究结果可为探明土著菌群与蚯蚓肠道菌群共享核心物种在抵御土壤中有机氯农药毒害作用的协同机制，提供新的科学认识。
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| 关 键 词： | 灭蚁灵 土著菌群 蚯蚓肠道菌群 核心物种 |
| 收稿时间： | 2020/10/24 0:00:00 |
| 修稿时间： | 2021/1/15 0:00:00 |
Keystone Species of Soil Indigenous Flora and Earthworm Intestinal Flora Help to Resist Mirex Stress |
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| ZHU Guofan,YING Rongrong,DENG Shaopo,KONG Lingy,QIAN Jiazhong,SUN Mingming,ZHU Yu,YE Mao.Keystone Species of Soil Indigenous Flora and Earthworm Intestinal Flora Help to Resist Mirex Stress[J].Soils,2021,53(6):1250-1260. |
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| Authors: | ZHU Guofan YING Rongrong DENG Shaopo KONG Lingy QIAN Jiazhong SUN Mingming ZHU Yu YE Mao |
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| Institution: | School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China;Nanjing Institute of Environmental Science, Ministry of Ecology and Environment, Nanjing 210042, China;College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;School of Politics & Public Administration, Hainan University, Haikou 570208, China; Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China |
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| Abstract: | Intis study soils with different mirex concentrations (0-27mg/kg) were collected to set up earthworm inoculation trials. High-throughput sequencing was used to analyze the structure and function of earthworm intestinal and soil indigenous microbial communities. Meanwhile, the keystone microbial taxa were identified through network analysis. The results obtained here found that 1) The structure and composition of the earthworm intestinal bacteria varied more significantly than those of the soil indigenous bacteria (P<0.05); 2) The keystone bacteria remained stable in the worm gut and the soil despite varying mirex concentration, which was mainly consisted of Aeromonas, Flavobacterium, Gallerella, Microvirga, Pedobacter, Ramlibacter, Zavarzinella; 3) The keystone bacteria had higher network connectivity than the rest bacteria, whose mean degree centrality, closeness centrality, eigenvector centrality values were 136.7, 0.44 and 0.52, respectively, clearly higher than those in the rest bacteria (91.52, 0.42 and 0.33, respectively). In addition, the keystone bacteria were closely involved in the carbon/nitrogen transformation and pesticide degradation, which not only protect the keystone bacteria from mirex toxicity, but might also assist other bacteria in relieving pesticide toxicity. The results obtained here shed novel light on understanding the mechanism of the earthworm intestinal and the soil indigenous bacteria in counteracting OCP stress and sustaining the homeostasis of microbial communities in the soil. |
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| Keywords: | Mirex Soil indigenous flora Earthworm intestinal flora Keystone taxa |
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