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| 绵羊体外成熟卵母细胞OPS法玻璃化冷冻保存试验 | |
| 引用本文： | 田树军,闫长亮,杨慧欣,杨中强,朱士恩.绵羊体外成熟卵母细胞OPS法玻璃化冷冻保存试验[J].中国畜牧杂志,2007,43(13):9-12. |
| 作者姓名： | 田树军 闫长亮 杨慧欣 杨中强 朱士恩 |
| 作者单位： | 1. 中国农业大学动物科技学院,北京,100094;河北农业大学河北省牛羊胚胎工程技术研究中心,河北保定,071001 2. 中国农业大学动物科技学院,北京,100094 3. 河北农业大学河北省牛羊胚胎工程技术研究中心,河北保定,071001 |
| 摘 要： | 研究以EDFS30为玻璃化冷冻液,以卵母细胞解冻后孤雌激活和体外受精后的卵裂率、囊胚发育率作为评价指标,探讨了以OPS法玻璃化冷冻保存体外成熟绵羊卵母细胞的效果。结果表明:卵母细胞孤雌激活后的卵裂率,冷冻组(64.2%)显著(P<0.05)低于毒性组(76.7%)和对照组(79.1%),而毒性组和对照组无显著(P>0.05)差异;卵母细胞孤雌激活后的囊胚发育率,冷冻组(4.2%)和毒性组(5.8%)均显著(P<0.05)低于对照组(20.2%),毒性组和冷冻组无显著(P>0.05)差异;冷冻组和毒性试验组卵母细胞体外受精后的卵裂率和囊胚发育率(67.6%和7.1%;62.3%和9.1%)均显著低于对照组(78.4%和28.4%)(P<0.05),而毒性组和冷冻组无显著(P>0.05)差异。可见以EDFS30为玻璃化冷冻液,采用OPS法冷冻保存绵羊体外成熟卵母细胞会在一定程度上降低其受精能力和胚胎发育能力。 |
| 关 键 词： | 卵母细胞 玻璃化冷冻 OPS 绵羊 |
| 文章编号： | 0258-7033（2007）13-0009-03 |
| 修稿时间： | 2006-09-062006-12-15 |
Effect of Vitrification in OPS on in vitro Maturated Ovine Oocyte's Developmental Potential |
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| TIAN Shu-jun,YAN Chang-liang,YANG Hui-xing,YANG Zhong-qiang,ZHU Shi-en.Effect of Vitrification in OPS on in vitro Maturated Ovine Oocyte''''s Developmental Potential[J].Chinese Journal of Animal Science,2007,43(13):9-12. | |
| Authors: | TIAN Shu-jun YAN Chang-liang YANG Hui-xing YANG Zhong-qiang ZHU Shi-en |
| Institution: | 1.College of Animal Science and Technology, China Agricultural University, Beijing 100094, China; 2.Hebei Province Embryo Engineering and Technology Research Center of Cattle and Sheep, Hebei Agricultural University, Hebei Baoding 071001, China |
| Abstract: | The present research was designed to investigate the effect of vitrification in EDFS30 by OPS on in vitro maturated ovine oocyte's developmental potential. Results were evaluated in terms of cleavage rate and blastocyst rate. The results showed that the parthenogenetically activated oocytes in vitrification group had a lower cleavage rates (64.2%) compared with toxicity group (76.7%) and control group (79.1%)(P < 0.05), there was no difference between the toxicity group and control group(P > 0.05); the blastocyst rates of parthenogenetically activated oocytes in vitrification group(4.2%) and toxicity group(5.8%) were decreased significantly than that of the control group (20.2%), and that was similar between the vitrification group and toxicity group(P > 0.05); the rates of cleavage and blastocyst of the in vitro fertilization oocytes in vitrification group (67.6% and 7.1%) and toxicity group (62.3% and 9.1%) were higher than that of control group (78.4% and 28.4%)(P < 0.05), and that was similar between the vitrification group and toxicity group(P > 0.05). These results indicated that the developmental potential of the oocytes vitrified in EDFS30 by OPS could be decreased in some extent. |
| Keywords: | OPS |
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