| 鸡传染性贫血病毒VP1和VP2基因共表达载体的构建 |
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| 引用本文： | 陈降华,周庆丰,刘忠华,李文平.鸡传染性贫血病毒VP1和VP2基因共表达载体的构建[J].动物医学进展,2009,30(11):68-71. |
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| 作者姓名： | 陈降华 周庆丰 刘忠华 李文平 |
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| 作者单位： | 1. 湖南农业大学动物医学院,湖南长沙,410128
2. 广东省温氏集团研究院,广东新兴,527400 |
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| 摘 要： | 根据GenBank中收录的鸡传染性贫血病毒(CIAV)基因组序列,设计了扩增CIAV VP1和VP2基因的特异性引物,运用PCR技术从发病鸡肝组织DNA中扩增得到VP1、VP2基因,并将其克隆至pMD18-T载体.核苷酸测序结果显示,其序列与发表的序列同源性达到99.8%.将VP2基因克隆到pMD18-T载体,并对Vp3基因起始密码子进行定点突变,得到突变基因mVP2.将mVP2、VP1先后克隆至pIRES载体,并转化大肠埃希菌JM109,提取质粒通过酶切鉴定显示,成功获得了共表达质粒pIRES-VP1/mVP2.
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| 关 键 词： | 鸡传染性贫血病毒 VP1基因 VP2基因 共表达载体 |
Construction of Chicken Infectious Anemia Virus Vector Based on VP1 and VP2 Genes |
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| CHEN Jiang-hua,ZHOU Qing-feng,LIU Zhong-hua,LI Wen-ping.Construction of Chicken Infectious Anemia Virus Vector Based on VP1 and VP2 Genes[J].Progress In Veterinary Medicine,2009,30(11):68-71. |
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| Authors: | CHEN Jiang-hua ZHOU Qing-feng LIU Zhong-hua LI Wen-ping |
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| Institution: | CHEN Jiang-hua1,ZHOU Qing-feng2,LIU Zhong-hua2,LI Wen-ping1(1.College of Veterinary Medicine,Hunan Agricultural University,Changsha,Hunan,410128,China,2.Guangdong Wen's Group academy,Xinxing,Guangdong,527400,China) |
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| Abstract: | Based on the sequences of infectious anemia gene published in the Genbank,two sets of specific primers were designed and synthesized.The genes of VP1 and VP2 were amplified by PCR from the DNA extracted from the infected chicken liver tissue,and then the VP1 and VP2 genes were cloned into the pMD18-T Simple vector.Sequencing analysis showed that the sequences of VP1 and VP2 were 99.8% homology with the published sequence.The mutated mVP2 gene was obtained after VP2 gene was cloned into the pMD18-T Simple ve... |
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| Keywords: | chicken infectious anemia virus VP1 and VP2 gene co-expression vector |
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