| 流式细胞术在活化T淋巴细胞检测中的应用 |
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| 引用本文： | 胡茂志,孟闯,潘志明,焦新安,刘秀梵.流式细胞术在活化T淋巴细胞检测中的应用[J].动物医学进展,2009,30(2). |
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| 作者姓名： | 胡茂志 孟闯 潘志明 焦新安 刘秀梵 |
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| 作者单位： | 1. 扬州大学江苏省人兽共患病学重点实验室,江苏扬州,225009;扬州大学江苏省物质微区与性能测试服务中心,江苏扬州,225009
2. 扬州大学江苏省人兽共患病学重点实验室,江苏扬州,225009 |
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| 基金项目： | 江苏省自然科学基金青年科技创新人才项目，江苏省高校自然科学基础研究项目 |
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| 摘 要： | 免疫细胞的活化和增殖是免疫应答产生的一种标志.静止T淋巴细胞经过抗原刺激活化后,表现出一系列的特征,如细胞膜表面活化分子的表达、细胞分裂和产生细胞因子等.近年来,已有利用流式细胞术(FCM)通过检测免疫细胞活化后的特性,来分析抗原特异性免疫应答及其应答规律的报道.论文分别从几个不同的角度综述了FCM在活化T淋巴细胞检测中的应用.
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| 关 键 词： | T淋巴细胞 活化 流式细胞术 |
Application of Flow Cytometry in the Detection of Activated T Lymphocytes |
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| HU Mao-zhi,MENG Chuang,PAN Zhi-ming,JIAO Xi-nan,LIU Xiu-fan.Application of Flow Cytometry in the Detection of Activated T Lymphocytes[J].Progress In Veterinary Medicine,2009,30(2). |
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| Authors: | HU Mao-zhi MENG Chuang PAN Zhi-ming JIAO Xi-nan LIU Xiu-fan |
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| Institution: | 1.Jiangsu Key Laboratory of Zoonesis;Yangzhou University;Yangzhou;225009;China;2.Jiangsu Test and Service Center for Material Microanalysis and Property;Yangzhou 225009;China |
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| Abstract: | The activation and proliferation of specific T lymphocytes can indicate the occurance of immune response.After being stimulated,the activated T lymphocytes may show some function characteristics,such as activation molecule expression on cell surface,cell proliferation,cytokine expression,and so on.Recently,there are some reports about the detection of antigen specific immune response through the analysis of activation function by using flow cytometry.This paper reviewed the application of flow cytometry in ... |
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| Keywords: | T lymphocytes activation flow cytometry |
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