| 利用C基因组对高杆野生稻和宽叶野生稻基因组的比较分析 |
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| 引用本文： | 刘凤麟,赵侯明,李刚,吴绮,覃瑞,刘虹.利用C基因组对高杆野生稻和宽叶野生稻基因组的比较分析[J].农业科学与技术,2009,10(3):11-14,21. |
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| 作者姓名： | 刘凤麟 赵侯明 李刚 吴绮 覃瑞 刘虹 |
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| 作者单位： | 中南民族大学生命科学学院,生物技术国家民委重点实验室,湖北武汉,430074 |
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| 摘 要： | 1材料与方法1．1植物材料和染色体制片药用野生稻稻株1589由广东省国家野生稻圃提供，宽叶野生稻IRW6和高杆野生稻IRW41由华南农业大学卢永根院士提供，试验材料情况见表1。染色体制片分别参照Yan等和Ren等的方法。
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| 关 键 词： | C基因组 高杆野生稻 宽叶野生稻基因组 染色体 |
Comparative Study on the Genomes Between Oryza alta and Oryza latifolia Based on C-Genome |
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| LIU Feng-lin,ZHAO Hou-ming,LI Gang,WU Qi,QIN Rui,LIU Hong.Comparative Study on the Genomes Between Oryza alta and Oryza latifolia Based on C-Genome[J].Agricultural Science ＆ Technology,2009,10(3):11-14,21. |
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| Authors: | LIU Feng-lin ZHAO Hou-ming LI Gang WU Qi QIN Rui LIU Hong |
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| Institution: | ( Key Laboratory for Siotechnology of the State Ethnic Affairs Commission, College of Life Science, South-Central University for Nationalities, Wuhan 430074) |
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| Abstract: | Objective] Genomic in situ hybridization (GISH) was used to study the relationship between the two CCDD genomes of Oryza alta and Oryza latifolia. Method] Total DNA of Oryza officinalis (C-genome) was used as a probe for genomic in situ hybridization on metaphase chromosomes from Oryza alta and Oryza latifolia, respectively. Result] Under certain post-hybridization washing stringencies, C- and D-genome could be distinguished in CCDD genome type; there were huge differences in some CC chromosomes of Oryza alta, Oryza latifolia, and Oryza officinalis. The genome of Oryza latifolia was more original. Conclusion] Comparative analysis of the Oryza species with identical genome type may facilitate to elucidate the possible approaches to plant genome evolution and species evolution. |
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| Keywords: | Oryza alta Oryza latifolia Genomic in situ hybridization |
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