| 水稻条斑和颖花异常双突变体的生理生化特性检测 |
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| 引用本文： | 陈德西,马炳田,王玉平,马玉清,李仕贵.水稻条斑和颖花异常双突变体的生理生化特性检测[J].农业科学与技术,2009,10(4):15-18. |
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| 作者姓名： | 陈德西 马炳田 王玉平 马玉清 李仕贵 |
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| 作者单位： | 陈德西(四川农业大学水稻研究所,四川温江611130;四川省农业科学院植保所,四川成都610066);马炳田,王玉平,马玉清,李仕贵(四川农业大学水稻研究所,四川温江,611130) |
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| 基金项目： | Program for New Century Excellent Talents in University，the Innovative Research Team in University (IRT0453).教育部新世纪优秀人才支持计划资助，教育部创新团队发展计划资助 |
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| 摘 要： | 笔者在转基因过程中发现1个条斑和颖花异常水稻双突变体，该突变体一些叶片细胞结构发生改变，叶绿素含量较野生型的减少，光合作用降低。为探究突变体叶片的生理生化变化以及对米质的影响，笔者就突变体生理生化进行研究，以期更清楚地了解突变体的突变机制，为进一步研究和利用该突变性状提供依据。
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| 关 键 词： | 生理生化变化 突变体 特性检测 异常 颖花 水稻 叶绿素含量 细胞结构 |
Physiological and Biochemical Characteristic of a Stripe Mutant with Abnormal Flower Organs in Rice |
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| CHEN De-xi,MA Bing-tian,WANG Yu-ping,MA Yu-qing,LI Shi-gui.Physiological and Biochemical Characteristic of a Stripe Mutant with Abnormal Flower Organs in Rice[J].Agricultural Science ＆ Technology,2009,10(4):15-18. |
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| Authors: | CHEN De-xi MA Bing-tian WANG Yu-ping MA Yu-qing LI Shi-gui |
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| Institution: | 1. Rice Research Institute, Sichuan Agricultural University, Chengdu 611130; 2. Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066) |
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| Abstract: | Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experimental materials to study the enzyme activity changes in different growth stages and amino acid variation in rice. Result] The SOD activity in mutant was higher than that in wild plant at tillering metaphase, but lower than that in wild type before heading stage and late flowering; the POD activity in three stages increased firstly then declined and the activity showed highest maximal activity at before heading stage. However, the POD activity in wild type showed the opposite change trend; the CAT activity presented degression at three stages, especially high at tillering metaphase, but reverse changes in wild type; the MDA activity decreased at three stages, but it was still higher than that in wild type, besides, the soluble sugar content of mutant was lower, but total amino acid content was increased. Conclusion] The expression of mutant characteristics was correlated with SOD, POD, CAT and MDA activity Changes and these changes made the mutant survive and rice quality change at last. |
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| Keywords: | Rice Stripe Physiological and Biochemical Characteristic Rice quality |
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