| ABA对‘巨峰’葡萄采后成熟关键基因表达的影响 |
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| 引用本文： | 张培安,王壮伟,蔡斌华,文习成,田亮,王晨,贾海锋,房经贵.ABA对‘巨峰’葡萄采后成熟关键基因表达的影响[J].园艺学报,2018,45(6):1067-1074. |
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| 作者姓名： | 张培安 王壮伟 蔡斌华 文习成 田亮 王晨 贾海锋 房经贵 |
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| 作者单位： | 1南京农业大学园艺学院，南京 210095；2江苏省农业科学院果树研究所，南京 210014 |
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| 基金项目： | 江苏省农业科技自主创新资金项目[CX（17）3031]；中央高校基本科研业务费专项资金项目（KYZ201736）；国家自然科学基金项目（31672131）；江苏省农业三新工程项目（SXGC[2017]278） |
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| 摘 要： | 利用ABA、NDGA(Nordihydroguaiaretictic acid,NCED酶抑制剂)和氟啶酮(Fluridone)、乙烯利和AS6(3’-hexylsulfanyl-ABA,PYL-PP2C受体拮抗剂)处理‘巨峰’葡萄采后果实,分析果实硬度、花色苷、芳香挥发物及相关基因表达量等的变化。结果表明,ABA可促进花色苷、可溶性固形物和香气挥发物形成,降低可滴定酸含量与果实硬度。NDGA、氟啶酮、AS6的效果与ABA相反。VvMYBA2、VvUFGT和VvEGS1的表达可促进花色苷的积累和芳香挥发物的形成,而Vv LAR1则减少花色苷积累。施加外源ABA,可促进果实内源ABA含量增加和VvOPR3、VvACO1和VvSUT11的表达;AS6处理也可增加内源性ABA含量,而NDGA、氟啶酮则相反。VvNCED1、VvCYP707A1、VvPYL8的表达与内源ABA含量基本一致,相比之下,AS6可下调V PYL8表达量。VvPP2C49的表达变化与内源ABA相反,AS6促进VvPP2C49表达。这些结果表明果实内源ABA含量影响花色苷、香气挥发产物的形成,促进果实软化和糖代谢。
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| 关 键 词： | 葡萄 果实 采后 ABA 成熟关键基因 |
Effects of ABA on the Expression of Key Genes in Postharvest Fruit of ‘Kyoho’Grapevine |
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| ZHANG Peian,WANG Zhuangwei,CAI Binhua,WEN Xicheng,TIAN Liang,WANG Chen,JIA Haifeng,FANG Jinggui.Effects of ABA on the Expression of Key Genes in Postharvest Fruit of ‘Kyoho’Grapevine[J].Acta Horticulturae Sinica,2018,45(6):1067-1074. |
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| Authors: | ZHANG Peian WANG Zhuangwei CAI Binhua WEN Xicheng TIAN Liang WANG Chen JIA Haifeng FANG Jinggui |
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| Institution: | Department of Cardiology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710048, China |
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| Abstract: | In order to reveal the senescence mechanism during fruit development，the effects of ABA，NDGA（Nordihydroguaiaretic acid，an inhibitor of NCED），fluridone，ethephon，and AS6（3′- hexylsulfanyl-ABA，antagonists of PYL-PP2C receptor）on fruit firmness，anthocyanin biosynthesis，aroma volatiles，and their related genes were investigated in grape（Vitis vinifera L. × V. labrusca L.‘Kyoho’）berries after postharvest. The results showed that ABA promoted anthocyanin，aroma volatile，and total soluble solid productions，but reduced fruit firmness and tartaric acid. Whereas NDGA，fluridone and AS6 showed an opposite effect. The expressions of VvMYBA2，VvUFGT or VvEGS1 coincided with anthocyanin content or aroma volatiles production，except that for the VvLAR1. Endogenous ABA content and the expressions of VvOPR3，VvACO1，and VvSUT11 were increased by ABA，but decreased by NDGA and fluridone. AS6 increased endogenous ABA content. The expressions of VvNCED1，VvCYP707A1 and VvPYL8 coincided with endogenous ABA content. In contrast，VvPYL8 expressions was decreased by AS6. The change of VvPP2C49 expression was contrary to ABA content，and AS6 increased VvPP2C49 expression. These results suggest that endogenous ABA content influence the anthocyanin formation，aroma volatile production，fruit softening，and sugar metabolism. |
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| Keywords: | grape fruit postharvest ABA key gene in postharvest |
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